EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.1 | phylogenetic tree, expression of GST-tagged LAP in Escherichia coli strain BL21, construction of a HA-tagged enzyme | Toxoplasma gondii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | bestatin | - |
Toxoplasma gondii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.1 | 0.01 | - |
L-phenylalanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.107 | - |
L-leucine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.124 | - |
L-proline-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.169 | - |
L-alanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.426 | - |
L-arginine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.11.1 | cytosol | - |
Toxoplasma gondii | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | Co2+ | activates | Toxoplasma gondii | |
3.4.11.1 | Mn2+ | activates, best metal ion | Toxoplasma gondii | |
3.4.11.1 | additional information | the enzyme activity strongly depends on metal ions, only slight activition by Fe2+, Mg2+, Ca2+, Cu2+, and Zn2+ | Toxoplasma gondii | |
3.4.11.1 | Ni2+ | activates | Toxoplasma gondii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 60000 | - |
x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE | Toxoplasma gondii |
3.4.11.1 | 85000 | - |
x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE | Toxoplasma gondii |
3.4.11.1 | 500000 | - |
recombinant enzyme, native PAGE | Toxoplasma gondii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.1 | Toxoplasma gondii | - |
- |
- |
3.4.11.1 | Toxoplasma gondii RH | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.1 | recombinant GST-tagged LAP from Escherichia coli strain BL21 by glutathione affinity chromatography | Toxoplasma gondii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.11.1 | tachyzoite | - |
Toxoplasma gondii | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.1 | L-alanine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-alanine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-alanine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-alanine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-arginine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-arginine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-arginine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-arginine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-leucine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-leucine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-phenylalanine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-phenylalanine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-phenylalanine-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-phenylalanine + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-proline-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii | L-proline + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.1 | L-proline-4-methylcoumarin-7-amide + H2O | - |
Toxoplasma gondii RH | L-proline + 7-amino-4-methylcoumarin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.1 | oligomer | x * 85000, recombinant GST-tagged enzyme, SDS-PAGE, x * 60000, native enzyme, SDS-PAGE | Toxoplasma gondii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.1 | LAP | - |
Toxoplasma gondii |
3.4.11.1 | leucine aminopeptidase | - |
Toxoplasma gondii |
3.4.11.1 | More | the enzyme belongs to the M17 family leucine aminopeptidases | Toxoplasma gondii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 37 | - |
assay at | Toxoplasma gondii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.1 | 0.0002 | - |
L-phenylalanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.0016 | - |
L-proline-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.03 | - |
L-alanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.041 | - |
L-arginine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.089 | - |
L-leucine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 8 | - |
assay at | Toxoplasma gondii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.1 | 0.0023 | - |
L-phenylalanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.013 | - |
L-proline-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.095 | - |
L-arginine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.18 | - |
L-alanine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii | |
3.4.11.1 | 0.832 | - |
L-leucine-4-methylcoumarin-7-amide | pH 8.0, 37°C, recombinant GST-tagged enzyme | Toxoplasma gondii |