EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.58 | gene WsEXG1, cloned from from both the genomic DNA and cDNA, DNA and amino acid sequence determination and analysis, subcloning of a His6-tagged enzyme in Escherichia coli strain DH5alpha, functional overexpression in Yarrowia lipolytica strain Po1h | Cyberlindnera saturnus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.58 | 1,10-phenanthroline | 40.5% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Ag+ | over 97% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Co2+ | 76.5% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Cu2+ | 87.7% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | EDTA | 99% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | EGTA | 94.5% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Fe2+ | 18.8% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Fe3+ | over 97% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Hg2+ | over 97% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | iodoacetic acid | 74.4% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Mn2+ | 87.5% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Ni2+ | 83.5% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | PMSF | 24% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | SDS | 34% inhibition at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | Zn2+ | 76% inhibition at 5 mM | Cyberlindnera saturnus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.58 | Ba2+ | activates 12% at 5 mM | Cyberlindnera saturnus | |
3.2.1.58 | additional information | no effect by 5 mM of Mg2+, Ca2+, Na+, K+, or Li+ | Cyberlindnera saturnus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.58 | 46000 | - |
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE | Cyberlindnera saturnus |
3.2.1.58 | 46200 | - |
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE | Cyberlindnera saturnus |
3.2.1.58 | 47500 | - |
x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE | Cyberlindnera saturnus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.58 | Cyberlindnera saturnus | C3VMY4 | i.e. Hansenula saturnus, a marine yeast, strain WC91-2, gene WsEXG1 | - |
3.2.1.58 | no activity in Yarrowia lipolytica | - |
- |
- |
3.2.1.58 | no activity in Yarrowia lipolytica Po1h | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.58 | recombinant His6-tagged enzyme 2.1fold from Yarrowia lipolytica strain Po1h to homogeneity | Cyberlindnera saturnus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.58 | 62 | - |
crude enzyme from wild-type strain WC91-2 | Cyberlindnera saturnus |
3.2.1.58 | 249 | - |
crude recombinant enzyme from overexpressing Yarrowia lipolytica strain Po1h | Cyberlindnera saturnus |
3.2.1.58 | 511 | - |
purified recombinant enzyme | Cyberlindnera saturnus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.58 | laminarin + H2O | active site glutamate residue | Cyberlindnera saturnus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.58 | ? | x * 46200, sequence calculation, x * 46000, recombinant enzyme, SDS-PAGE, x * 47500, native enzyme, SDS-PAGE | Cyberlindnera saturnus |
3.2.1.58 | More | enzyme model with active site glutamate residue, overview | Cyberlindnera saturnus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.58 | exo-beta-1,3-glucanase | - |
Cyberlindnera saturnus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.58 | 40 | - |
- |
Cyberlindnera saturnus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.58 | 30 | 55 | activity range | Cyberlindnera saturnus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.58 | 40 | - |
purified recombinant enzyme, stable up to | Cyberlindnera saturnus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.58 | 5 | - |
- |
Cyberlindnera saturnus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.58 | 3 | 9.5 | recombinant enzyme, 4°C, 2 h, stable | Cyberlindnera saturnus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.58 | Cyberlindnera saturnus | sequence calculation | - |
4.5 |