EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.4 | drug development | new pharmaceutical approaches against PLA2 activity can be designed to prevent either membrane adsorption or nonhydrolytic bilayer-disrupting activity, both of which are necessary for enzymatic activity, opening up a new set of specific functional targets for inhibitor design | Apis mellifera |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.4 | Ca2+ | free Ca2+ is required for interaction between PLA2 and vesicles under catalytic conditions, Ca2+ addition for activation of PLA2 under initially noncatalytic conditions. Differences between enzymatic activity under initially catalytic and noncatalytic conditions, membrane binding under noncatalytic conditions must either cause PLA2 to adopt a less favorable conformation for enzymatic activity or prevent a Ca2+ ion (after Ca2+ addition) from entering the active site pocket | Apis mellifera |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.4 | Apis mellifera | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.4 | venom | - |
Apis mellifera | - |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.4 | 4°C, 10 mM Tris buffer, pH 7.7, 150 mM NaCl, 2 mM EDTA | Apis mellifera |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.4 | additional information | PLA2 shows low enzymatic activity toward positively charged vesicles due to a long lag period. PLA2 demonstrates a high degree of enzymatic activity toward negatively charged 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine/1,2-dioleoyl-sn-glycero-3-phosphatidylserine vesicles. Vesicle size distribution of zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine changes faster than that of 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine/1,2-dioleoyl-sn-glycero-3-phosphatidylserine 90:10 | Apis mellifera | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.4 | secretory phospholipase A2 | - |
Apis mellifera |
3.1.1.4 | sPLA2 | - |
Apis mellifera |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.1.4 | physiological function | adsorption of PLA2 to model membranes is not primarily driven by electrostatic interactions. Subsequent lipid desorption, which is linked to the bilayer-disrupting activity of PLA2, is significantly affected by membrane electrostatics. Specifically, a nonhydrolytic bilayer-disrupting activity of PLA2 targets anionic membranes, triggering a change in bilayer topology | Apis mellifera |