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Literature summary extracted from

  • Bansal, V.; Delgado, Y.; Fasoli, E.; Ferrer, A.; Griebenow, K.; Secundo, F.; Barletta, G.L.
    Effect of prolonged exposure to organic solvents on the active site environment of subtilisin Carlsberg (2010), J. Mol. Catal. B, 64, 38-44.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 (dimethylamino)-1-naphthalenesulfonyl fluoride 100% inhibition of enzyme activity with N-trans cinnamoyl imidazole as substrate, during prolonged exposure to organic solvents the active-site fluorescent label inhibitor adopts a different binding conformation Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus licheniformis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 additional information substrates bind in a less catalytically favorable conformation after the enzyme has been exposed to organic media for several hours Bacillus licheniformis ?
-
 ?
3.4.21.62 N-trans-cinnamoyl imidazole + H2O
-
 Bacillus licheniformis (E)-cinnamate + imidazole
-
 ?
3.4.21.62 sec-phenethyl alcohol + vinyl butyrate
-
 Bacillus licheniformis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 subtilisin C.
-
 Bacillus licheniformis
3.4.21.62 subtilisin Carlsberg
-
 Bacillus licheniformis

General Information

EC Number General Information Comment Organism
3.4.21.62 physiological function when subtilisin C. is chemically modified with polyethylene glycol and inhibited with a dansyl fluorophore, and initially dissolved in two organic solvents (acetonitrile and 1,4-dioxane), the active site environment of the enzyme is similar to that in water. Prolonged exposure to the organic medium causes this environment to resemble that of the solvent in which the enzyme is dissolved Bacillus licheniformis