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Literature summary extracted from

  • Chanjuan, L.; Hong, Y.; Shao, Z.; Lin, L.; Huang, X.; Liu, P.; Wu, G.; Meng, X.; Liu, Z.
    Novel alkali-stable, cellulase-free xylanase from deep-sea Kocuria sp. Mn22 (2009), J. Microbiol. Biotechnol., 19, 873-880.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene Kxyn, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21 (DE3) Kocuria sp. Mn22

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Co2+
-
 Kocuria sp. Mn22
3.2.1.8 Cu2+
-
 Kocuria sp. Mn22
3.2.1.8 Fe2+
-
 Kocuria sp. Mn22
3.2.1.8 Hg2+
-
 Kocuria sp. Mn22
3.2.1.8 Mn2+
-
 Kocuria sp. Mn22
3.2.1.8 additional information Kxyn is not affected by 2-mercaptoethanol, DTT, or SDS Kocuria sp. Mn22
3.2.1.8 Pb2+
-
 Kocuria sp. Mn22
3.2.1.8 Zn2+
-
 Kocuria sp. Mn22

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Km for beta-1,4-xylan from birchwood is 5.4 mg/ml Kocuria sp. Mn22

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 additional information Kxyn is not affected by Ca2+, Mg2+, Na+, K+ Kocuria sp. Mn22

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Kocuria sp. Mn22
-
 xylobiose + xylotriose + ? predominant products ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Kocuria sp. Mn22 B8XY24 gene Kxyn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant enzyme from Escherichia coli strain BL21 (DE3) Kocuria sp. Mn22

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Kocuria sp. Mn22 xylobiose + xylotriose + ? predominant products ?
3.2.1.8 beta-1,4-xylan + H2O from birchwood Kocuria sp. Mn22 xylobiose + xylotriose + ? predominant products ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endo-beta-1,4-xylanase
-
 Kocuria sp. Mn22
3.2.1.8 More the enzyme belongs to the glycosyl hydrolase family 10, GH10 Kocuria sp. Mn22
3.2.1.8 xylanase
-
 Kocuria sp. Mn22

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 55
-
-
 Kocuria sp. Mn22

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 30 70 activity range Kocuria sp. Mn22

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 55
-
 purified recombinant enzyme, 60 min, 70% activity remaining Kocuria sp. Mn22
3.2.1.8 60
-
 purified recombinant enzyme, 60 min, 30% activity remaining Kocuria sp. Mn22
3.2.1.8 70
-
 purified recombinant enzyme, 60 min, 10% activity remaining Kocuria sp. Mn22

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 185.1
-
 beta-1,4-xylan pH 8.5, 55°C, birchwood xylan Kocuria sp. Mn22

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 8.5
-
-
 Kocuria sp. Mn22

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 6 12 activity range Kocuria sp. Mn22

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 7.6 12 purified recombinant enzyme, over 80% remaining activity after overnight incubation Kocuria sp. Mn22