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Literature summary extracted from

  • Kim, H.S.; Kim, J.Y.; Park, M.S.; Zheng, H.; Ji, G.E.
    Cloning and expression of beta-glucuronidase from Lactobacillus brevis in E. coli and application in the bioconversion of baicalin and wogonoside (2009), J. Microbiol. Biotechnol., 19, 1650-1655.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.31 GUS, DNA and amino acid sequence determination, analysis and comparison, overexpression of the His-tagged enzyme in Escherichia coli strain GMS407 Levilactobacillus brevis
3.2.1.167 expressed in Escherichia coli strain GMS407 (beta-glucuronidase-deficient) Levilactobacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 71000
-
 x * 71000, recombinant enzyme, SDS-PAGE Levilactobacillus brevis
3.2.1.167 71000
-
 recombinant enzyme, SDS-PAGE Levilactobacillus brevis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Levilactobacillus brevis
-
-
-
3.2.1.31 Levilactobacillus brevis RO1
-
-
-
3.2.1.167 Levilactobacillus brevis D2DMF3 strain RO1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31 recombinant GUS from Escherichia coli strain GMS407 Levilactobacillus brevis
3.2.1.167 Ni-NTA column chromatography Levilactobacillus brevis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 1284
-
 purified recombinant enzyme Levilactobacillus brevis
3.2.1.167 1.284
-
 at 37°C and pH 5.0, using 4-nitrophenyl-beta-D-glucuronide as substrate Levilactobacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O
-
 Levilactobacillus brevis 4-nitrophenol + beta-D-glucuronate
-
 ?
3.2.1.31 4-nitrophenyl beta-D-glucuronide + H2O
-
 Levilactobacillus brevis RO1 4-nitrophenol + beta-D-glucuronate
-
 ?
3.2.1.31 baicalin + H2O
-
 Levilactobacillus brevis baicalein + beta-D-glucuronate
-
 ?
3.2.1.31 baicalin + H2O
-
 Levilactobacillus brevis RO1 baicalein + beta-D-glucuronate
-
 ?
3.2.1.31 wogonin 7-O-beta-D-glucuronide + H2O
-
 Levilactobacillus brevis wogonin + beta-D-glucuronate
-
 ?
3.2.1.31 wogonin 7-O-beta-D-glucuronide + H2O
-
 Levilactobacillus brevis RO1 wogonin + beta-D-glucuronate
-
 ?
3.2.1.167 4-nitrophenyl beta-D-glucuronide + H2O
-
 Levilactobacillus brevis 4-nitrophenol + beta-D-glucuronic acid
-
 ?
3.2.1.167 baicalin + H2O 0.15 mM of baicalin is completely transformed into baicalein within 3 h at 37°C, pH 5.0 Levilactobacillus brevis baicalein + D-glucuronate
-
 ?
3.2.1.167 wogonoside + H2O 0.125 mM of wogonoside is completely transformed into wogonin within 3 h at 37°C, pH 5.0 Levilactobacillus brevis wogonin + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.31 ? x * 71000, recombinant enzyme, SDS-PAGE Levilactobacillus brevis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.31 GUS
-
 Levilactobacillus brevis
3.2.1.31 More the enzyme belongs to glycosyl hydrolase family 2 with three conserved domains Levilactobacillus brevis
3.2.1.167 beta-glucuronidase
-
 Levilactobacillus brevis
3.2.1.167 GUS
-
 Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.31 37
-
 recombinant enzyme Levilactobacillus brevis
3.2.1.167 37
-
-
 Levilactobacillus brevis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.31 37
-
 80 min, purified recombinant enzyme, pH 5.0-8.0, completely stable Levilactobacillus brevis
3.2.1.31 50
-
 purified recombinant enzyme, half-life is over 2 h Levilactobacillus brevis
3.2.1.31 60
-
 purified recombinant enzyme, half-life is 1 h Levilactobacillus brevis
3.2.1.167 50 60 the purified enzyme exhibits a half-life of 1 h at 60°C and more than 2 h at 50°C Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 5
-
 recombinant enzyme Levilactobacillus brevis
3.2.1.167 5
-
-
 Levilactobacillus brevis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.31 5 8 80 min, purified recombinant enzyme, 37°C, completely stable Levilactobacillus brevis
3.2.1.167 5 8 at 37°C, the GUS remains stable for 80 min at pH values ranging from 5.0 to 8.0. Activity decreases quickly above pH 7.0 Levilactobacillus brevis