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Literature summary extracted from

  • Sajjad, M.; Khan, M.I.; Akbar, N.S.; Ahmad, S.; Ali, I.; Akhtar, M.W.
    Enhanced expression and activity yields of Clostridium thermocellum xylanases without non-catalytic domains (2010), J. Biotechnol., 145, 38-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xynC, subcloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli Acetivibrio thermocellus
3.2.1.8 gene xynZ, subcloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli Acetivibrio thermocellus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 additional information generation of two constructs of XynC, one with its cellulose binding domain and the catalytic domain, pXynC-BC, and the other with only the catalytic domain, pXynC-C. The specific activities of XynC with and without the non-catalytic domains are similar Acetivibrio thermocellus
3.2.1.8 additional information generation of two constructs of XynZ: pXynZ-BDC, which includes the dockerin domain, and pXynZ-C, which does not. For XynZ, the specific activity of the enzyme without the non-catalytic domains is about 5fold greater than that of the intact enzyme. The overall increase in activity is 9fold higher for XynZ-C versus XynZ-BDC Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Km values for mutants XynC-BC and XynC-C determined on soluble xylan are 3.1 mg/ml and 3.6 mg/ml, respectively Acetivibrio thermocellus
3.2.1.8 additional information
-
 additional information Km values for mutants XynZ-BDC and XynZ-C, determined on soluble xylan, are 33.3 mg/ml and 15.4 mg/ml, respectively Acetivibrio thermocellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Acetivibrio thermocellus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Acetivibrio thermocellus O32374 xynC; strain ATCC 27405D, gene xynC
-
3.2.1.8 Acetivibrio thermocellus P10478 xynZ; strain ATCC 27405D, gene xynZ
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
 activities of wild-type XynC, and its domain deletion mutants, overview Acetivibrio thermocellus
3.2.1.8 additional information
-
 activities of wild-type XynZ, and its domain deletion mutants, overview Acetivibrio thermocellus
3.2.1.8 3 8 purified mutant XynC-BC Acetivibrio thermocellus
3.2.1.8 39
-
 purified mutant XynC-C Acetivibrio thermocellus
3.2.1.8 79
-
 purified mutant XynZ-BDC Acetivibrio thermocellus
3.2.1.8 390
-
 purified mutant XynZ-C Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O from birchwood Acetivibrio thermocellus ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O insoluble xylan from birchwood, and soluble xylan Acetivibrio thermocellus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Acetivibrio thermocellus
3.2.1.8 XynC
-
 Acetivibrio thermocellus
3.2.1.8 XynZ
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 30
-
 assay at Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
 2 h, 87% activity of mutant Xyn-BDC is remaining, 42% of mutant Xyn-BDC Acetivibrio thermocellus
3.2.1.8 70
-
 2 h, mutant Xyn-C loses all activity, while mutant XynC-BC retains 70% activity Acetivibrio thermocellus