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Literature summary extracted from

  • Suzuki, S.; Fukuoka, M.; Ookuchi, H.; Sano, M.; Ozeki, K.; Nagayoshi, E.; Takii, Y.; Matsushita, M.; Tada, S.; Kusumoto, K.; Kashiwagi, Y.
    Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli (2010), J. Biosci. Bioeng., 109, 115-117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expression in Escherichia coli Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.48
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0 Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 37400
-
 x * 37400, calculated Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Aspergillus oryzae Q2URT3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein sequence contains a potential N-glycosylation site at N243 Aspergillus oryzae
3.2.1.37 additional information sequence contains no potential secretion signal peptides Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenol + D-xylose
-
 ?
3.2.1.37 additional information no substrate: 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside and 4-nitrophenyl-beta-D-mannopyranoside Aspergillus oryzae ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Aspergillus oryzae 2 D-xylose
-
 ?
3.2.1.37 xylotriose + H2O
-
 Aspergillus oryzae 3 D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 37400, calculated Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 XylB
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 30
-
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 30
-
 up to, gradual inactivation Aspergillus oryzae
3.2.1.37 40
-
 drastic inactivation above Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 7
-
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 7 9 more than 60% of maximum activity Aspergillus oryzae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.37 Aspergillus oryzae calculated
-
 4.8