Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wu, B.; Yin, J.; Texier, C.; Roussel, M.; Tan, K.S.
    Blastocystis legumain is localized on the cell surface, and specific inhibition of its activity implicates a pro-survival role for the enzyme (2010), J. Biol. Chem., 285, 1790-1798.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 glutathione S-transferase-fusion protein expressed in Escherichia coli BL21(DE3) Blastocystis sp. BW-2009a
3.4.22.34 glutathione S-transferase-fusion protein expressed in Escherichia coli BL21(DE3) Blastocystis hominis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 carbobenzyloxy-Ala-Ala-(aza-Asn)-epoxycarboxylate ethyl ester
-
 Blastocystis hominis
3.4.22.34 carbobenzyloxy-Ala-Ala-(aza-Asn)-epoxycarboxylate ethyl ester
-
 Blastocystis sp. BW-2009a

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 30000
-
 x * 30000, 2-D electrophoresis Blastocystis sp. BW-2009a
3.4.22.34 30000
-
 x * 30000, 2-D electrophoresis Blastocystis hominis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Blastocystis hominis D1M8Z4
-
-
3.4.22.34 Blastocystis sp. BW-2009a C1K3M8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34 affinity chromatography (glutathione-Sepharose) Blastocystis sp. BW-2009a
3.4.22.34 affinity chromatography (glutathione-Sepharose) Blastocystis hominis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
 Blastocystis sp. BW-2009a benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
 Blastocystis hominis benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 ? x * 30000, 2-D electrophoresis Blastocystis sp. BW-2009a
3.4.22.34 ? x * 30000, 2-D electrophoresis Blastocystis hominis

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 legumain
-
 Blastocystis sp. BW-2009a
3.4.22.34 legumain
-
 Blastocystis hominis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 7.5
-
 biphasic pH optima with peaks at pH 4 (about 70% activity) and pH 7.5 (100% activity) Blastocystis sp. BW-2009a
3.4.22.34 7.5
-
 biphasic pH optima with peaks at pH 4 (about 70% activity) and pH 7.5 (100% activity) Blastocystis hominis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.34 3.5 8
-
 Blastocystis sp. BW-2009a
3.4.22.34 3.5 8
-
 Blastocystis hominis