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Literature summary extracted from

  • Alvaro-Benito, M.; Polo, A.; Gonzalez, B.; Fernandez-Lobato, M.; Sanz-Aparicio, J.
    Structural and kinetic analysis of Schwanniomyces occidentalis invertase reveals a new oligomerization pattern and the role of its supplementary domain in substrate binding (2010), J. Biol. Chem., 285, 13930-13941.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.26 three-dimensional structure of invertase at 2.9 A resolution and its complex with fructose at 1.9 A resolution. The monomer presents a bimodular arrangement common to other GH32 enzymes, with an N-terminal 5fold beta-propeller catalytic domain and a C-terminal alpha-sandwich domain. Structure displays an unique active site cleft shaped by both subunits. Medium size and long substrates are recognized by residues from both subunits Schwanniomyces occidentalis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.26 D50A no catalytic activity Schwanniomyces occidentalis
3.2.1.26 E230A no catalytic activity Schwanniomyces occidentalis
3.2.1.26 N142Y large decrease in activity against nystose Schwanniomyces occidentalis
3.2.1.26 N254A large decrease in activity against nystose Schwanniomyces occidentalis
3.2.1.26 Q176E moderate change in catalytic activity Schwanniomyces occidentalis
3.2.1.26 Q176S moderate change in catalytic activity Schwanniomyces occidentalis
3.2.1.26 Q228V strikingly large reduction in the catalytic efficiency of sucrose , inulin and especially nystose Schwanniomyces occidentalis
3.2.1.26 Q435A significant decrease in catalytic efficiency against inulin Schwanniomyces occidentalis
3.2.1.26 S281I strikingly large reduction in the catalytic efficiency of sucrose , inulin and especially nystose Schwanniomyces occidentalis
3.2.1.26 Y462A significant decrease in catalytic efficiency against inulin Schwanniomyces occidentalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 2.4
-
 sucrose wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 3.8
-
 nystose mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 5
-
 sucrose mutant Q176S, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 6.4
-
 sucrose wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 7
-
 inulin mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 7
-
 sucrose mutant Q176E, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 7
-
 sucrose mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 8
-
 nystose mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 8
-
 sucrose mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 8
-
 nystose mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 8
-
 nystose mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 8.1
-
 nystose mutant Q176E, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 9
-
 nystose mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 9
-
 sucrose mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 9
-
 inulin mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 11
-
 nystose mutant Q176S, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 11
-
 sucrose mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 12
-
 inulin wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 13
-
 sucrose mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 14
-
 inulin mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 15
-
 nystose mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 24
-
 inulin mutant Q176E, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 29
-
 sucrose mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 44
-
 inulin mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 47
-
 inulin mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 60
-
 inulin mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 575
-
 inulin mutant Q176S, pH 5.0 Schwanniomyces occidentalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 extracellular
-
 Schwanniomyces occidentalis
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Schwanniomyces occidentalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 inulin + H2O
-
 Schwanniomyces occidentalis D-fructose + ?
-
 ?
3.2.1.26 nystose + H2O
-
 Schwanniomyces occidentalis D-fructose + D-glucose
-
 ?
3.2.1.26 sucrose + H2O
-
 Schwanniomyces occidentalis D-fructose + D-glucose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 2
-
 nystose mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 2
-
 inulin mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 3
-
 inulin mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 5
-
 inulin mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 6
-
 nystose mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 10
-
 inulin mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 12
-
 inulin mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 20
-
 sucrose mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 22
-
 nystose mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 25
-
 inulin wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 33
-
 sucrose mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 39
-
 inulin mutant Q176E, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 43
-
 sucrose mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 60
-
 nystose mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 68
-
 inulin mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 76
-
 nystose mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 84
-
 sucrose wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 85
-
 sucrose mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 87
-
 nystose mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 88
-
 sucrose mutant Q176E, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 92
-
 nystose mutant Q176E, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 99
-
 sucrose mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 106
-
 sucrose wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 134
-
 sucrose mutant Q176S, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 230
-
 nystose mutant Q176S, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 262
-
 sucrose mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 1710
-
 inulin mutant Q176S, pH 5.0 Schwanniomyces occidentalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.26 0.04
-
 inulin mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 0.06
-
 inulin mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 0.2
-
 nystose mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 0.4
-
 nystose mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 0.5
-
 inulin mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 0.7
-
 inulin mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 1.1
-
 inulin mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 1.5
-
 sucrose mutant S281I, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 1.6
-
 inulin mutant Q176E, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 1.7
-
 inulin mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 2.1
-
 inulin wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 2.7
-
 nystose mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 3
-
 inulin mutant Q176S, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 3
-
 sucrose mutant Q228V, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 6
-
 sucrose mutant Y462A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 7.5
-
 nystose mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 9
-
 sucrose mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 10.6
-
 sucrose mutant N254A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 11
-
 nystose mutant N142Y, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 11
-
 sucrose mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 12.6
-
 sucrose mutant Q176E, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 16.6
-
 sucrose wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 20
-
 nystose mutant Q435A, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 21
-
 nystose mutant Q176S, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 26.8
-
 sucrose mutant Q176S, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 35
-
 sucrose wild-type, pH 5.0 Schwanniomyces occidentalis
3.2.1.26 51
-
 nystose mutant Q176E, pH 5.0 Schwanniomyces occidentalis