BRENDA - Enzyme Database

Sterol-mediated regulation of human lipin 1 gene expression in hepatoblastoma cells

Ishimoto, K.; Nakamura, H.; Tachibana, K.; Yamasaki, D.; Ota, A.; Hirano, K.; Tanaka, T.; Hamakubo, T.; Sakai, J.; Kodama, T.; Doi, T.; J. Biol. Chem. 284, 22195-22205 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
LPIN1 reporter plasmid transiently transfected into Hep-G2 cells
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
Huh-7 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
709037
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
LPIN1 reporter plasmid transiently transfected into Hep-G2 cells
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
Huh-7 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
709037
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
3.1.3.4
Homo sapiens
LPIN1 mRNA levels decrease significantly after transfection with LPIN1 siRNA. LPIN1 mRNA levels are reduced to ca. 0.6fold after transfection with SREBP-1 siRNA
down
3.1.3.4
Homo sapiens
LPIN1 mRNA does not change in cells transfected with SREBP-2 siRNA. Both the sterol regulatory element and the nuclear factor Y-binding site are important in the regulation of LPIN1 transcription
additional information
3.1.3.4
Homo sapiens
sterol regulatory element-binding protein 1 (SREBP-1) and nuclear factor Y are necessary for the activation of LPIN1 transcription. Induction of the LPIN1 gene by sterol depletion. The region between nucleotides -2428 and -2378 of the LPIN1 promoter contains sequences that mediate the induction of LPIN1 by sterol depletion. LPIN1 mRNA levels are induced 3.3fold in lipoprotein-deficient serum medium relative to those in sterol-containing medium. In statin-containing medium, LPIN1 mRNA increases 7.2fold
up
General Information
EC Number
General Information
Commentary
Organism
3.1.3.4
physiological function
sterol-mediated regulation of lipin 1 gene transcription modulates triglyceride accumulation
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.3.4
physiological function
sterol-mediated regulation of lipin 1 gene transcription modulates triglyceride accumulation
Homo sapiens
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.1.3.4
Homo sapiens
LPIN1 mRNA levels decrease significantly after transfection with LPIN1 siRNA. LPIN1 mRNA levels are reduced to ca. 0.6fold after transfection with SREBP-1 siRNA
down
3.1.3.4
Homo sapiens
LPIN1 mRNA does not change in cells transfected with SREBP-2 siRNA. Both the sterol regulatory element and the nuclear factor Y-binding site are important in the regulation of LPIN1 transcription
additional information
3.1.3.4
Homo sapiens
sterol regulatory element-binding protein 1 (SREBP-1) and nuclear factor Y are necessary for the activation of LPIN1 transcription. Induction of the LPIN1 gene by sterol depletion. The region between nucleotides -2428 and -2378 of the LPIN1 promoter contains sequences that mediate the induction of LPIN1 by sterol depletion. LPIN1 mRNA levels are induced 3.3fold in lipoprotein-deficient serum medium relative to those in sterol-containing medium. In statin-containing medium, LPIN1 mRNA increases 7.2fold
up