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Literature summary extracted from

  • Schoijet, A.C.; Miranda, K.; Girard-Dias, W.; de Souza, W.; Flawia, M.M.; Torres, H.N.; Docampo, R.; Alonso, G.D.
    A Trypanosoma cruzi phosphatidylinositol 3-kinase (TcVps34) is involved in osmoregulation and receptor-mediated endocytosis (2008), J. Biol. Chem., 283, 31541-31550.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.137 DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged TcVps34 in Escherichia coli strain BL21(DE3) and complementation of an enzyme-deficient Saccharomyces cerevisiae knockout mutant strain Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.137 additional information in TcVps34-overexpressing cells, the activities of vacuolar-H+-ATPase and vacuolar H+-pyrophosphatase are altered, suggesting defects in the acidification of intracellular compartments, phenotype, overview. Overexpressing cells show resistance to PI3K inhibitors. TcVps34-overexpressing epimastigotes show morphological alterations in the plasma membrane region between the cytostome and the flagellar pocket Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.137 LY-294,002
-
 Trypanosoma cruzi
2.7.1.137 additional information inhibition of TcVps34 with specific PI3K inhibitors, such as wortmannin and LY294000, result in reduced regulatory volume decrease after hyposmotic stress Trypanosoma cruzi
2.7.1.137 Wortmannin
-
 Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.137 plasma membrane
-
 Trypanosoma cruzi 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.137 ATP + 1-phosphatidyl-1D-myo-inositol Trypanosoma cruzi TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.1.137 ATP + 1-phosphatidyl-1D-myo-inositol Trypanosoma cruzi CL Brener TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.137 Trypanosoma cruzi A9QWR8 CL Brener strain
-
2.7.1.137 Trypanosoma cruzi CL Brener A9QWR8 CL Brener strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.137 recombinant His-tagged TcVps34 from Escherichia coli strain BL21(DE3) by affinity chromatography Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.137 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.137 ATP + 1-phosphatidyl-1D-myo-inositol TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate Trypanosoma cruzi ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.1.137 ATP + 1-phosphatidyl-1D-myo-inositol TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate Trypanosoma cruzi CL Brener ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.137 More TcVps34 belongs to the class III PI3K family Trypanosoma cruzi
2.7.1.137 PI3K
-
 Trypanosoma cruzi
2.7.1.137 TcVps34
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.137 ATP
-
 Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
2.7.1.137 malfunction inhibition of TcVps34 with specific PI3K inhibitors, such as wortmannin and LY294000, result in reduced regulatory volume decrease after hyposmotic stress Trypanosoma cruzi
2.7.1.137 physiological function the Trypanosoma cruzi phosphatidylinositol 3-kinase, TcVps34, is involved in osmoregulation and receptor-mediated endocytosis. TcVps34 participates in the endocytic pathway, fluid phase uptake of BSA and receptor-mediated endocytosis of transferrin. Multiple roles for TcVps34, schematic overview Trypanosoma cruzi