Literature summary extracted from
Cianci, M.; Tomaszewski, B.; Helliwell, J.R.; Halling, P.J.
Crystallographic analysis of counterion effects on subtilisin enzymatic action in acetonitrile (2010), J. Am. Chem. Soc., 132, 2293-2300.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.4.21.62 |
choline |
increases the enzyme activity |
Bacillus licheniformis |
|
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.4.21.62 |
by the batch method from buffer solution saturated with Na2SO4 ca. 13% (w/v) as precipitant. Placement of several Cs+ and Cl- ions in crystals of subtilisin Carlsberg. The protein conformation is very similar to that of the enzyme without CsCl in acetonitrile. 11 defined sites for Cs+ cations and 8 Cl- anions around the protein molecule, although most of these have partial occupancy and may represent nonspecific binding sites. Two Cs+ and two Cl- ions are close to the mouth of the active site cleft, where they may affect catalysis. CsCl-treated subtilisin crystals transferred to acetonitrile show catalytic activity several fold higher than the reference crystals containing Na+ |
Bacillus licheniformis |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.21.62 |
Cl- |
treatment with CsCl increases the activity several fold. Two Cl- ions are close to the mouth of the active site cleft, where they may affect catalysis |
Bacillus licheniformis |
|
3.4.21.62 |
Cs+ |
treatment with CsCl increases the activity several fold. Two Cs+ ions are close to the mouth of the active site cleft, where they may affect catalysis |
Bacillus licheniformis |
|
3.4.21.62 |
KCl |
increases enzymatic activity somewhat, while choline-Cl has a larger effect |
Bacillus licheniformis |
|
3.4.21.62 |
additional information |
enzymatic activity of subtilisin crystals in acetonitrile is sensitive to the type of counterions present before transfer to the organic solvent. Larger cations increase the enzyme activity |
Bacillus licheniformis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.21.62 |
Bacillus licheniformis |
P00780 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.21.62 |
N-acetyl-Tyr ethyl ester + H2O |
- |
Bacillus licheniformis |
N-acetyl-Tyr + ethanol |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.21.62 |
subtilisin Carlsberg |
- |
Bacillus licheniformis |