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Literature summary extracted from
- Heterologous expression and biochemical characterization of alpha-glucosidase from Aspergillus niger by Pichia pastroris (2010), J. Agric. Food Chem., 58, 4819-4824.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | expression in Pichia pastoris | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 0.446 | - |
p-nitrophenyl-alpha-D-glucopyranoside | - |
Aspergillus niger |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 106000 | - |
SDS-PAGE, after deglycosylation | Aspergillus niger |
| 3.2.1.20 | 145000 | - |
SDS-PAGE | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Aspergillus niger | A2QJF7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.20 | ultrafiltration, ethanol fraction and hydrophobic interaction chromatography | Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 2.52 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | assay at pH 5.5, 50°C, reaction terminated by sodium carbonate solution | Aspergillus niger | 4-nitrophenol + D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.20 | alpha-glucosidase | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 60 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 30 | 60 | - |
Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 50 | - |
50% remaining activity after 72 h at 50°C, 50% remaining activity after 3 h at 60°C | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 4.5 | - |
- |
Aspergillus niger |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 3 | 7 | - |
Aspergillus niger |
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Release 2023.1 (January 2023)
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