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Literature summary extracted from

  • Weaver, J.D.; Ullah, A.H.; Sethumadhavan, K.; Mullaney, E.J.; Lei, X.G.
    Impact of assay conditions on activity estimate and kinetics comparison of Aspergillus niger PhyA and Escherichia coli AppA2 phytases (2009), J. Agric. Food Chem., 57, 5315-5320.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.8 analysis comparison of assay methods and the impact of assay conditions on activity estimate using the molybdenum blue method, the molybdovanadate method, and the acetone phosphomolybdate method. Nearly identical activity of PhyA is determined from the molybdenum blue method and the acetone phosphomolybdate method. The molybdenum blue method and the molybdovanadate method give only 22% difference in PhyA activity Aspergillus niger
3.1.3.8 analysis comparison of assay methods and the impact of assay conditions on activity estimate using the molybdenum blue method, the molybdovanadate method, and the acetone phosphomolybdate method. The activity values of AppA2 are more variable with the three assay methods than those of Aspergillus niger PhyA. The molybdenum blue method and the molybdovanadate method produce nearly a 3fold disparity for AppA2. Overall, the pH value, the type of buffers, and the inclusion of ancillary chemicals such as the detergents Triton X-100 and BSA each account for approximately one-third of the variations of AppA2 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Pichia pastoris Escherichia coli
3.1.3.8 expression in Pichia pastoris Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.034
-
 myo-inositol hexakisphosphate pH 3.5, 37°C Aspergillus niger
3.1.3.8 0.074
-
 myo-inositol hexakisphosphate pH 3.5, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 47000
-
 x * 47000, SDS-PAGE Escherichia coli
3.1.3.8 84000
-
 x * 84000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger
-
-
-
3.1.3.8 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein
-
 Escherichia coli
3.1.3.8 glycoprotein
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Escherichia coli 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus niger 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 47000, SDS-PAGE Escherichia coli
3.1.3.8 ? x * 84000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 AppA2
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 170
-
 myo-inositol hexakisphosphate pH 3.5, 37°C Aspergillus niger
3.1.3.8 840
-
 myo-inositol hexakisphosphate pH 3.5, 37°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.8 5000
-
 myo-inositol hexakisphosphate pH 3.5, 37°C Aspergillus niger
3.1.3.8 11400
-
 myo-inositol hexakisphosphate pH 3.5, 37°C Escherichia coli