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Literature summary extracted from

  • Fazary, A.E.; Hamad, H.A.; Lee, J.C.; Koskei, T.; Lee, C.K.; Ju, Y.H.
    Expression of feruloyl esterase from Aspergillus awamori in Escherichia coli: Characterization and crystal studies of the recombinant enzyme (2010), Int. J. Biol. Macromol., 46, 440-444.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 PCR products ligated to pTZ57R/T cloning vector and expressed in competent Escherichia coli TBL21 strain Aspergillus awamori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.73 by hanging drop vapor diffusion method at 20°C, to 2.5 A resolution. Crystals of recombinant FaeA enzyme and its complex with ferulic acid belong to the triclinic space group P212121, with unit cell dimensions of a = 40.04, b = 48.98, c = 61.98 A and alpha = 98.65, beta = 93.98, gamma = 103.98, and a = 41.04, b = 49.28, c = 62.23 A and alpha = 98.01, beta = 92.12, gamma = 100.11, respectively Aspergillus awamori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 1.38
-
 ethyl ferulate
-
 Aspergillus awamori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 35000
-
 x * 35000, SDS-PAGE Aspergillus awamori

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus awamori Q9P979
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 by Ni-NTA affinity chromatography and gel filtration, with 19.57% yield Aspergillus awamori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 1.85
-
 purified enzyme with a yield of 19.54% Aspergillus awamori

Storage Stability

EC Number Storage Stability Organism
3.1.1.73 -70°C Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 ethyl ferulate + H2O
-
 Aspergillus awamori ferulic acid + ethanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 35000, SDS-PAGE Aspergillus awamori

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAEA
-
 Aspergillus awamori
3.1.1.73 feruloyl esterase
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 55
-
-
 Aspergillus awamori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 25 75 more than 20% of the activity is retained after heating the enzyme at 85°C Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5.5
-
-
 Aspergillus awamori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 4 9 enzyme loses more than 50% of its activity when incubated at pH less than 3.5. About 40% of the activity remains after being treated at pH below 4.0 or over 8.0 Aspergillus awamori

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Aspergillus awamori
-
-
 4.2