Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fuchise, T.; Kishimura, H.; Sekizaki, H.; Nonami, Y.; Kanno, G.; Klomklao, S.; Benjakul, S.; Chun, B.
    Purification and characteristics of trypsins from cold-zone fish, Pacific cod (Gadus macrocephalus) and saffron cod (Eleginus gracilis) (2009), Food Chem., 116, 611-616.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.4 Ca2+ stabilizes Gadus macrocephalus
3.4.21.4 Ca2+ stabilizes Eleginus gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 additional information no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA Eleginus gracilis
3.4.21.4 additional information no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA Gadus macrocephalus
3.4.21.4 Nalpha-tosyl-L-Lys-chloromethylketone 79% inhibition at 5 mM Eleginus gracilis
3.4.21.4 Nalpha-tosyl-L-Lys-chloromethylketone 89% inhibition at 5 mM Gadus macrocephalus
3.4.21.4 Soybean trypsin inhibitor 84% inhibition at 1 mg/ml Eleginus gracilis
3.4.21.4 Soybean trypsin inhibitor 80% inhibition at 1 mg/ml Gadus macrocephalus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.4 Ca2+ stabilizes Gadus macrocephalus
3.4.21.4 Ca2+ stabilizes Eleginus gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 24000
-
 x * 24000, SDS-PAGE Gadus macrocephalus
3.4.21.4 24000
-
 x * 24000, SDS-PAGE Eleginus gracilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Eleginus gracilis
-
-
-
3.4.21.4 Gadus macrocephalus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 native enzyme 28fold from pyloric ceca to homogeneity by gel filtration Eleginus gracilis
3.4.21.4 native enzyme 33fold from pyloric ceca to homogeneity by gel filtration Gadus macrocephalus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 cecum
-
 Gadus macrocephalus
-
3.4.21.4 cecum
-
 Eleginus gracilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.4 18
-
 purified enzyme, pH 8.0, 50°C Eleginus gracilis
3.4.21.4 24
-
 purified enzyme, pH 8.0, 50°C Gadus macrocephalus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 additional information the enzyme from catayzes also the reverse reaction and synthesizes Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide from Nalpha-(tert-butoxycarbonyl)-L-alanine-4-guanidinophenyl ester as acyl donor and L-alanine-p-nitroanilide (L-Ala-pNA) as acyl acceptor, overview Gadus macrocephalus ?
-
 ?
3.4.21.4 Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide + H2O
-
 Gadus macrocephalus Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine + 4-nitroaniline
-
 ?
3.4.21.4 Nalpha-p-tosyl-L-arginine methyl ester + H2O
-
 Gadus macrocephalus ?
-
 ?
3.4.21.4 Nalpha-p-tosyl-L-arginine methyl ester + H2O
-
 Eleginus gracilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.4 ? x * 24000, SDS-PAGE Gadus macrocephalus
3.4.21.4 ? x * 24000, SDS-PAGE Eleginus gracilis

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 EG-T
-
 Eleginus gracilis
3.4.21.4 GM-T
-
 Gadus macrocephalus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.4 50
-
-
 Gadus macrocephalus
3.4.21.4 50
-
-
 Eleginus gracilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.4 20 70 activity range Gadus macrocephalus
3.4.21.4 20 70 activity range Eleginus gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.4 30
-
 purified enzyme, unstable above Gadus macrocephalus
3.4.21.4 30
-
 purified enzyme, unstable above Eleginus gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 8
-
-
 Gadus macrocephalus
3.4.21.4 8
-
-
 Eleginus gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.4 7 9 high activity within this range Gadus macrocephalus
3.4.21.4 7 9 high activity within this range Eleginus gracilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.4 5 10 purified enzyme, unstable below, completely stable at pH 6.0-10.0 for 30 min Eleginus gracilis
3.4.21.4 5 10 purified enzyme, unstable below, completely stable at pH 7.0-10.0 for 30 min Gadus macrocephalus