EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.28 | expression of GFP-tagged Ath1 N-terminal domain | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.28 | additional information | complementation of the Saccharomyces cerevisiae suc2D mutant SEY6210 strain with different Ath1-invertase chimera, where the Suc2 signal peptide is replaced by full-length ATH1 sequence, overview. Hybrid Ath1 truncated mutants fused at their C-terminus with the yeast internal invertase reveal that a 131 amino acid N-terminal fragment of Ath1 is sufficient to target the fusion protein to the cell surface, enabling growth of the suc2DELTA mutant on sucrose. Removal of the N-terminus of Ath1 causes a strict vacuolar localization. Fusion of the signal peptide of invertase to N-terminally truncated Ath1 allows the ath1D mutant to grow on trehalose, whereas the signal sequence of the vacuolar-targeted Pep4 constrained Ath1 in the vacuole and prevents growth of this mutant on trehalose | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.28 | cell surface | Ath1 | Saccharomyces cerevisiae | 9986 | - |
3.2.1.28 | additional information | Ath1 possesses a transmembrane domain. Existence of a secretion sequence in Ath1 | Saccharomyces cerevisiae | - |
- |
3.2.1.28 | vacuole | Ath1 | Saccharomyces cerevisiae | 5773 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 30000 | - |
x * 30000, about, recombinant GFP-tagged Ath1 N-terminal domain | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | alpha,alpha-trehalose + H2O | Saccharomyces cerevisiae | trehalose is one of the major storage carbohydrates in the yeast Saccharomyces cerevisiae | 2 D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.28 | Saccharomyces cerevisiae | - |
neutral Nth1 and Nth2 and acid Ath1, encoded by gene ath1 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Saccharomyces cerevisiae | 2 D-glucose | - |
? | |
3.2.1.28 | alpha,alpha-trehalose + H2O | trehalose is one of the major storage carbohydrates in the yeast Saccharomyces cerevisiae | Saccharomyces cerevisiae | 2 D-glucose | - |
? | |
3.2.1.28 | additional information | the 131 amino acid N-terminus of Ath1 are sufficient for invertase secretion, and the short transmembrane domain, located at the N-terminus, is indispensable for Ath1 function, overview | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.28 | ? | x * 30000, about, recombinant GFP-tagged Ath1 N-terminal domain | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.28 | Ath1 | - |
Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 4.5 | - |
Ath1 | Saccharomyces cerevisiae |