BRENDA - Enzyme Database

Catalytic properties of an expressed and purified higher plant type zeta-carotene desaturase from Capsicum annuum

Breitenbach, J.; Kuntz, M.; Takaichi, S.; Sandmann, G.; Eur. J. Biochem. 265, 376-383 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.5.6
decyl-plastoquinone
enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate
Capsicum annuum
1.3.5.6
decyl-ubiquinone
enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate
Capsicum annuum
Cloned(Commentary)
EC Number
Commentary
Organism
1.3.5.6
expression in Escherichia coli
Capsicum annuum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.6
0.0084
-
zeta-carotene
pH 7.8, 28°C
Capsicum annuum
1.3.5.6
0.009
-
Neurosporene
pH 7.8, 28°C
Capsicum annuum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.6
60000
-
x * 60000, SDS-PAGE
Capsicum annuum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.6
neurosporene + a quinone
Capsicum annuum
-
lycopene + a quinol
-
-
?
1.3.5.6
zeta-carotene + 2 quinone
Capsicum annuum
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
lycopene + 2 quinol
-
-
?
1.3.5.6
zeta-carotene + a quinone
Capsicum annuum
-
neurosporene + a quinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.5.6
Capsicum annuum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.5.6
-
Capsicum annuum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.6
neurosporene + a quinone
-
708367
Capsicum annuum
lycopene + a quinol
-
-
-
?
1.3.5.6
neurosporene + decyl-plastoquinone
-
708367
Capsicum annuum
lycopene + decyl-plastoquinol
-
-
-
?
1.3.5.6
neurosporene + decyl-ubiquinone
-
708367
Capsicum annuum
lycopene + decyl-ubiquinone
-
-
-
?
1.3.5.6
zeta-carotene + 2 decyl-plastoquinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 decyl-plastoquinol
-
-
-
?
1.3.5.6
zeta-carotene + 2 decyl-ubiquinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 decyl-ubiquinone
-
-
-
?
1.3.5.6
zeta-carotene + 2 quinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 quinol
-
-
-
?
1.3.5.6
zeta-carotene + a quinone
-
708367
Capsicum annuum
neurosporene + a quinol
-
-
-
?
1.3.5.6
zeta-carotene + decyl-plastoquinone
-
708367
Capsicum annuum
neurosporene + decyl-plastoquinol
-
-
-
?
1.3.5.6
zeta-carotene + decyl-ubiquinone
-
708367
Capsicum annuum
neurosporene + decyl-ubiquinone
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.5.6
?
x * 60000, SDS-PAGE
Capsicum annuum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.5.6
28
-
assay at
Capsicum annuum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.6
7.8
-
assay at
Capsicum annuum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.6
additional information
addition of the dinucleotides FAD, NADP+ or NAD+ alone or the latter in combination with ATP results in a negligible increase in zeta-carotene desaturase activity
Capsicum annuum
1.3.5.6
quinone
-
Capsicum annuum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.5.6
decyl-plastoquinone
enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate
Capsicum annuum
1.3.5.6
decyl-ubiquinone
enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate
Capsicum annuum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.6
expression in Escherichia coli
Capsicum annuum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.5.6
additional information
addition of the dinucleotides FAD, NADP+ or NAD+ alone or the latter in combination with ATP results in a negligible increase in zeta-carotene desaturase activity
Capsicum annuum
1.3.5.6
quinone
-
Capsicum annuum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.5.6
0.0084
-
zeta-carotene
pH 7.8, 28°C
Capsicum annuum
1.3.5.6
0.009
-
Neurosporene
pH 7.8, 28°C
Capsicum annuum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.5.6
60000
-
x * 60000, SDS-PAGE
Capsicum annuum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.5.6
neurosporene + a quinone
Capsicum annuum
-
lycopene + a quinol
-
-
?
1.3.5.6
zeta-carotene + 2 quinone
Capsicum annuum
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
lycopene + 2 quinol
-
-
?
1.3.5.6
zeta-carotene + a quinone
Capsicum annuum
-
neurosporene + a quinol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.5.6
-
Capsicum annuum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.5.6
neurosporene + a quinone
-
708367
Capsicum annuum
lycopene + a quinol
-
-
-
?
1.3.5.6
neurosporene + decyl-plastoquinone
-
708367
Capsicum annuum
lycopene + decyl-plastoquinol
-
-
-
?
1.3.5.6
neurosporene + decyl-ubiquinone
-
708367
Capsicum annuum
lycopene + decyl-ubiquinone
-
-
-
?
1.3.5.6
zeta-carotene + 2 decyl-plastoquinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 decyl-plastoquinol
-
-
-
?
1.3.5.6
zeta-carotene + 2 decyl-ubiquinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 decyl-ubiquinone
-
-
-
?
1.3.5.6
zeta-carotene + 2 quinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 quinol
-
-
-
?
1.3.5.6
zeta-carotene + a quinone
-
708367
Capsicum annuum
neurosporene + a quinol
-
-
-
?
1.3.5.6
zeta-carotene + decyl-plastoquinone
-
708367
Capsicum annuum
neurosporene + decyl-plastoquinol
-
-
-
?
1.3.5.6
zeta-carotene + decyl-ubiquinone
-
708367
Capsicum annuum
neurosporene + decyl-ubiquinone
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.5.6
?
x * 60000, SDS-PAGE
Capsicum annuum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.3.5.6
28
-
assay at
Capsicum annuum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.5.6
7.8
-
assay at
Capsicum annuum