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Literature summary extracted from

  • Goto, M.; Harada, Y.; Oka, T.; Matsumoto, S.; Takegawa, K.; Furukawa, K.
    Protein O-mannosyltransferases B and C support hyphal development and differentiation in Aspergillus nidulans (2009), Eukaryot. Cell, 8, 1465-1474.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.109 PCR-amplification, introduction of replacement cassettes to disrupt genes Aspergillus nidulans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.109 additional information Anpmta + AnpmtB double disruptant is viable but very slow growing with morphological characteristics (swollen hyphae with ballon structures + hyperbranched hyphae) cumulative of single disruptants at 30 and 42°C, partial improvement of defects by addition of osmotic stabilizer Aspergillus nidulans
2.4.1.109 additional information AnpmtA disruptant, increased sensitivity to hygromycin B (increased cell wall permeability), glycoprotetin profile (plasma membrane proteins) almost indistinguishable from wild-type, underglycosylation of protein AN5660 upon AnpmtC diruption Aspergillus nidulans
2.4.1.109 additional information AnPmtB disruptant shows wild-type colony formation at 30°C, slightly repressed growth at 42°C, the osmotic stabilizer 0.6 M KCl reduces these effects, conidiation reduced to about 56%, swollen vesicles, hyperbranching of hyphae (defect in polarity maintenance), underglycosylation of glucoamylase I upon gene disruption, higher sensitivity to inhibitors of cell wall synthesis (beta-glucan and beta-1,3-glucan production) congo red and micafungin, not to calcofluor (inhibiting chitin synthesis), no increased sensitivity to hygromycin B, glycoproetin profile (plasma membrane proteins) almost indistinguishable from wild-type, underglycosylation of glucoamylase I upon AnpmtB diruption Aspergillus nidulans
2.4.1.109 additional information AnpmtC disruptant with highest growth repression, swollen, frequently branched hyphae, strongly reduced conidia formation (6% of wild-type), recovery of hyphal structures in the presence of osmotic stabilizers (0.6 M KCl, 0.8 M NaCl, or 1.2 M sorbitol) at 42°C, enables conidiophore and conidia production (abnormal and fewer), underglycosylation of glucoamylase I upon gene diruption, higher sensitivity to inhibitors of cell wall synthesis (beta-glucan and beta-1,3-glucan production) congo red and micafungin, not to calcofluor (inhibiting chitin synthesis), more sensitive to hygromycin B than wild-type, increase of 65 kDa proteins, of mannose-containing glycoproteins (plasma membrane proteins) of about 80 kDa, reduced number of proteins larger than 100 kDa and of 75 kDa, underglycosylation of glucoamylase I and of protein AN5660 upon AnpmtC diruption Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.109 88200
-
 AnPmtC, calculated from amino acid sequence Aspergillus nidulans
2.4.1.109 103300
-
 AnPmtB, calculated from amino acid sequence Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.109 Aspergillus nidulans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + glucoamylase I extracellular Aspergillus awamori protein as reporter for glycosylation measurements, underglycosylation upon AnpmtA, AnpmtB, and AnpmtC diruption Aspergillus nidulans dolichyl phosphate + O-D-mannosyl-[glucoamylase]
-
 ?
2.4.1.109 dolichyl phosphate D-mannose + protein AN5660 triple-hemagglutin-tagged AN5660.3 protein is an ortholog of Wsc1 protein of Saccharomyces cerevisiae, a sensor for cell wall stress, underglycosylation upon AnpmtA and AnpmtC diruption not due to AnpmtB disruption Aspergillus nidulans dolichyl phosphate + O-D-mannosylprotein AN5660 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.109 heterodimer AnpmtB with AnpmtA, 2 * ? Aspergillus nidulans
2.4.1.109 heterodimer AnpmtB with AnpmtA, but AnPmtB disruption mutant shows different phenotype than AnpmtA disruptant, independent function Aspergillus nidulans
2.4.1.109 homodimer
-
 Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.109 AnPmtA PMT2 subfamily Aspergillus nidulans
2.4.1.109 AnPmtB PMT1 subfamily Aspergillus nidulans
2.4.1.109 AnPmtC PMT4 subfamily Aspergillus nidulans
2.4.1.109 O-mannosyltransferase
-
 Aspergillus nidulans

General Information

EC Number General Information Comment Organism
2.4.1.109 malfunction AnpmtA + AnpmtB double disruptant is viable but slow growing with morphological characteristics cumulative of single disruptants Aspergillus nidulans
2.4.1.109 malfunction AnpmtA + AnpmtB double disruptant is viable but slow growing with morphological characteristics cumulative of single disruptants, hyperglycosylation of specific target proteins Aspergillus nidulans
2.4.1.109 malfunction AnpmtB disruptant shows wild-type colony formation at 30°C, slightly repressed growth at 42°C, conidiation reduced to about 50%, hyperbranching of hyphae (defect in polarity maintenance) Aspergillus nidulans
2.4.1.109 malfunction pmtC disruptant with highest growth repression, swollen, frequently branched hyphae, no conidia formation, recovery of hyphal structures in the presence of osmotic stabilizers, enables conidiophore and conidia production (abnormal and fewer) at 42°C Aspergillus nidulans
2.4.1.109 physiological function AnpmtB, hyphal development and differentiation, polarity maintenance Aspergillus nidulans
2.4.1.109 physiological function AnpmtC, hyphal development and differentiation, maintenance of cell wall integrity Aspergillus nidulans
2.4.1.109 physiological function fungal morphology and conidia formation Aspergillus nidulans