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Literature summary extracted from

  • Encinar del Dedo, J.; Duenas, E.; Arnaiz, Y.; del Rey, F.; Vazquez de Aldana, C.R.
    beta-glucanase Eng2 is required for ascus wall endolysis after sporulation in the fission yeast Schizosaccharomyces pombe (2009), Eukaryot. Cell, 8, 1278-1286.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 placing of gene eng2+ under the control of a repressible promoter Schizosaccharomyces pombe
3.2.1.39 placing of gene eng2+ under the control of a repressible promoter Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.6 additional information a point mutation, that destroys the catalytic activity of the protein, results in a phenotype similar to that of the mutant strain with gene eng2+ placed under the control of a repressible promoter. Exogenous addition of purified Eng2 releases the ascospores from asci generated by the eng2+-deficient mutant Schizosaccharomyces pombe
3.2.1.39 additional information a point mutation, that destroys the catalytic activity of the protein, results in a phenotype similar to that of the mutant strain with gene eng2 placed under the control of a repressible promoter. Exogenous addition of purified Eng2 releases the ascospores from asci generated by the eng2+-deficient mutant Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.6 cytosol Eng2 lacks a signal peptide for entry into the secretory pathway and therefore localizes intracellularly to the cytosol of the diploid cell Schizosaccharomyces pombe 5829
-
3.2.1.39 cytosol Eng2 lacks a signal peptide for entry into the secretory pathway and therefore localizes intracellularly to the cytosol of the diploid cell Schizosaccharomyces pombe 5829
-
3.2.1.59 cytosol Agn2 lacks a signal peptide for entry into the secretory pathway and therefore localizes intracellularly to the cytosol of the diploid cell Schizosaccharomyces pombe 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.6 beta-glucan + H2O Schizosaccharomyces pombe
-
 ?
-
 ?
3.2.1.6 laminarin + H2O Schizosaccharomyces pombe
-
 ?
-
 ?
3.2.1.39 beta-glucan + H2O Schizosaccharomyces pombe
-
 ?
-
 ?
3.2.1.39 laminarin + H2O Schizosaccharomyces pombe
-
 ?
-
 ?
3.2.1.59 alpha-1,3-glucan + H2O Schizosaccharomyces pombe
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Schizosaccharomyces pombe
-
 several strains, overview
-
3.2.1.39 Schizosaccharomyces pombe
-
 several strains, overview
-
3.2.1.59 Schizosaccharomyces pombe
-
 several strains, overview, gene agn2+
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.6 cell culture Eng2 is present during the mitotic cycle, and accumulates after meiosis II, expression of eng2+ is required for the efficient release of ascospores, expression of eng2+ is highly upregulated during the sporulation process, and the product is mainly involved in spore morphogenesis Schizosaccharomyces pombe
-
3.2.1.39 cell culture Eng2 is present during the mitotic cycle, and accumulates after meiosis II, expression of eng2+ is required for the efficient release of ascospores, expression of eng2+ is highly upregulated during the sporulation process, and the product is mainly involved in spore morphogenesis Schizosaccharomyces pombe
-
3.2.1.59 cell culture expression of agn2+ is highly upregulated during the sporulation process, and the product is mainly involved in spore morphogenesis Schizosaccharomyces pombe
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 beta-glucan + H2O
-
 Schizosaccharomyces pombe ?
-
 ?
3.2.1.6 laminarin + H2O
-
 Schizosaccharomyces pombe ?
-
 ?
3.2.1.39 beta-glucan + H2O
-
 Schizosaccharomyces pombe ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Schizosaccharomyces pombe ?
-
 ?
3.2.1.59 alpha-1,3-glucan + H2O
-
 Schizosaccharomyces pombe ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 beta-glucanase
-
 Schizosaccharomyces pombe
3.2.1.6 Eng2
-
 Schizosaccharomyces pombe
3.2.1.6 More the enzyme belongs to the glycosyl hydrolase family 81, GH81 Schizosaccharomyces pombe
3.2.1.39 beta-glucanase
-
 Schizosaccharomyces pombe
3.2.1.39 Eng2
-
 Schizosaccharomyces pombe
3.2.1.39 More the enzyme belongs to the glycosyl hydrolase family 81, GH81 Schizosaccharomyces pombe
3.2.1.59 Agn2
-
 Schizosaccharomyces pombe
3.2.1.59 endo-alpha-1,3-glucanase
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 37
-
 assay at Schizosaccharomyces pombe
3.2.1.39 37
-
 assay at Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5.5
-
 assay at Schizosaccharomyces pombe
3.2.1.39 5.5
-
 assay at Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
3.2.1.6 physiological function When Schizosaccharomyces pombe diploid cells undergo meiosis, they differentiate into asci containing four haploid ascospores that are highly resistant to environmental stress. beta-Glucanase Eng2 and endo-alpha-1,3-glucanase Agn2 are required for ascus wall endolysis after sporulation in Schizosaccharomyces pombe Schizosaccharomyces pombe
3.2.1.39 physiological function When Schizosaccharomyces pombe diploid cells undergo meiosis, they differentiate into asci containing four haploid ascospores that are highly resistant to environmental stress. beta-Glucanase Eng2 and endo-alpha-1,3-glucanase Agn2 are required for ascus wall endolysis after sporulation in Schizosaccharomyces pombe Schizosaccharomyces pombe
3.2.1.59 physiological function When Schizosaccharomyces pombe diploid cells undergo meiosis, they differentiate into asci containing four haploid ascospores that are highly resistant to environmental stress. beta-Glucanase Eng2 and endo-alpha-1,3-glucanase Agn2 are required for ascus wall endolysis after sporulation in Schizosaccharomyces pombe Schizosaccharomyces pombe