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Literature summary extracted from

  • Mozolowski, G.; Connerton, I.
    Characterization of a highly efficient heterodimeric xylosidase from Humicola insolens (2009), Enzyme Microb. Technol., 45, 436-442.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.37 Triton X-100 10-20% stimulation Humicola insolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Cu2+ 90% inhibition Humicola insolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 1.74
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.6, 40°C Humicola insolens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 Ca2+ 10-20% stimulation Humicola insolens
3.2.1.37 Mg2+ 10-20% stimulation Humicola insolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 17000
-
 1 * 68000, catalytic subunit, 1 * 17000, carbohydrate binding subunit, SDS-PAGE Humicola insolens
3.2.1.37 68000
-
 1 * 68000, catalytic subunit, 1 * 17000, carbohydrate binding subunit, SDS-PAGE Humicola insolens
3.2.1.37 84000
-
 analytical ultracentrifugation Humicola insolens
3.2.1.37 92200
-
 sedimentation velocity analysis Humicola insolens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Humicola insolens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37
-
 Humicola insolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Humicola insolens 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.37 additional information no substrate: 4-nitrophenyl-alpha-L-arabinofuranoside Humicola insolens ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Humicola insolens 2 D-xylose
-
 ?
3.2.1.37 xylotriose + H2O
-
 Humicola insolens 3 D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 dimer 1 * 68000, catalytic subunit, 1 * 17000, carbohydrate binding subunit, SDS-PAGE Humicola insolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 70
-
-
 Humicola insolens