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Literature summary extracted from

  • Izquierdo, L.; Schulz, B.L.; Rodrigues, J.A.; Guether, M.L.; Procter, J.B.; Barton, G.J.; Aebi, M.; Ferguson, M.A.
    Distinct donor and acceptor specificities of Trypanosoma brucei oligosaccharyltransferases (2009), EMBO J., 28, 2650-2661.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.99.18 medicine enzyme inhibition could have therapeutic potential against trypanosomiasis Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.18 PCR-amplification, TbSTT3A expression in Saccharomyces cerevisiae does not replace yeast SST3 Trypanosoma brucei
2.4.99.18 PCR-amplification, TbSTT3B expression in Saccharomyces cerevisiae can replace yeast SST3, mild temperature-sensitive growth at 37°C, wild-type growth at 23°C Trypanosoma brucei
2.4.99.18 PCR-amplification, TbSTT3C expression in Saccharomyces cerevisiae can replace yeast SST3, severe growth defects at 23°C Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.99.18 RNAi silencing of TbSTT3A Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine Trypanosoma brucei
-
 dolichyl disphosphate + protein with oligosaccharide attached to protein L-asparagine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.18 Trypanosoma brucei
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.99.18 additional information TbSTT3A transcribed in the bloodstream nor procyclic form Trypanosoma brucei
-
2.4.99.18 additional information TbSTT3B transcribed in the bloodstream nor procyclic form Trypanosoma brucei
-
2.4.99.18 additional information TbSTT3C not transcribed in the bloodstream nor procyclic form Trypanosoma brucei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.18 biantennary Man5GlcNAc2 dolichyl disphosphate + VSG221 coat glycoprotein selective transfer of TbSTT3A to acidic to neutral regions of polypeptides, responsible for all paucimannose and complex N-glycans in Trypanosoma brucei glycoproteins Trypanosoma brucei dolichyl disphosphate + ?
-
 ?
2.4.99.18 biantennary Man5GlcNAc2 dolichyl disphosphate + VSG221 coat glycoprotein selective transfer of TbSTT3A to acidic to neutral regions of polypeptides, responsible for all paucimannose and complex N-glycans in Trypanosoma brucei glycoproteins Trypanosoma brucei dolichyl disphosphate + protein with oligosaccharide attached to protein L-asparagine
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine
-
 Trypanosoma brucei dolichyl disphosphate + protein with oligosaccharide attached to protein L-asparagine
-
 ?
2.4.99.18 triantennary Man9GlcNAc2 dolichyl disphosphate + VSG221 coat glycoprotein selective transfer of TbSTT3B to neutral to basic regions of polypeptides responsible for most or all oligomannose N-glycans in Trypanosoma brucei glycoproteins Trypanosoma brucei dolichyl disphosphate + protein with oligosaccharide attached to protein L-asparagine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.99.18 oligosaccharyltransferase
-
 Trypanosoma brucei
2.4.99.18 OTase
-
 Trypanosoma brucei
2.4.99.18 TbSTT3A
-
 Trypanosoma brucei
2.4.99.18 TbSTT3B
-
 Trypanosoma brucei
2.4.99.18 TbSTT3C
-
 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
2.4.99.18 malfunction simultaneous TbSTT3A and TbSTT3B knockdown prevents parasite growth in the bloodstream Trypanosoma brucei
2.4.99.18 physiological function TbSTT3A and TbSTT3B N-glycosylation together is essential for infectivity in mice Trypanosoma brucei