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Literature summary extracted from

  • Deng, H.; O'Hagan, D.
    The fluorinase, the chlorinase and the duf-62 enzymes (2008), Curr. Opin. Chem. Biol., 12, 582-592.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.B21 expression in Escherichia coli Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.94 G131S the mutation results in instability and arrested chlorination Salinispora tropica
2.5.1.94 Y70T the mutation leads to a 1000fold loss of chlorination activity Salinispora tropica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.B21 S-adenosyl-L-methionine + H2O Pyrococcus horikoshii the enzyme is most active at pH 8.5, so perhaps it has a regulatory role in influencing the cellular pH in the host organism. Many, but not all of the duf-62 containing organisms are extremophiles adenosine + L-methionine + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.B21 Pyrococcus horikoshii O58212
-
-
2.5.1.94 Salinispora tropica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.B21 S-adenosyl-L-methionine + H2O the enzyme is most active at pH 8.5, so perhaps it has a regulatory role in influencing the cellular pH in the host organism. Many, but not all of the duf-62 containing organisms are extremophiles Pyrococcus horikoshii adenosine + L-methionine + H+
-
 ?
2.5.1.B21 S-adenosyl-L-methionine + H2O mechanistic hypothesis for SAM hydroxide adenosyltransferase Pyrococcus horikoshii adenosine + L-methionine + H+
-
 ?
2.5.1.94 additional information although this enzyme utilises bromide and even iodide it does not fluorinate Salinispora tropica ?
-
 ?
2.5.1.94 S-adenosyl-L-methionine + chloride
-
 Salinispora tropica 5'-deoxy-5'-chloroadenosine + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.94 homotrimer x-ray crystallography Salinispora tropica

Synonyms

EC Number Synonyms Comment Organism
2.5.1.B21 duf-62
-
 Pyrococcus horikoshii
2.5.1.B21 SAM hydroxide adenosyltransferase
-
 Pyrococcus horikoshii
2.5.1.94 chlorinase
-
 Salinispora tropica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.B21 37
-
 assay at Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.B21 8.5
-
-
 Pyrococcus horikoshii

General Information

EC Number General Information Comment Organism
2.5.1.94 malfunction a chlorinase knockout mutated strain of Salinispora tropica is able to biosynthesis the fluorinated analogue of salinosporamide A, when the medium was supplemented with 5-deoxy-5’-fluoroadenosine Salinispora tropica