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Literature summary extracted from

  • Song, J.M.; Nam, K.; Sun, Y.U.; Kang, M.H.; Kim, C.G.; Kwon, S.T.; Lee, J.; Lee, Y.H.
    Molecular and biochemical characterizations of a novel arthropod endo-beta-1,3-glucanase from the Antarctic springtail, Cryptopygus antarcticus, horizontally acquired from bacteria (2010), Comp. Biochem. Physiol. B, 155, 403-412.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 gene CaLam, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis and tree, recombinant expression of His-tagged and thioredoxin-fused enzyme without signal sequence in Escherichia coli strain Rosetta(DE3), subcloning in Escherichia coli strain DH5alpha Cryptopygus antarcticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 additional information kinetics Cryptopygus antarcticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 28983
-
 x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE Cryptopygus antarcticus
3.2.1.39 46000
-
 x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE Cryptopygus antarcticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 laminarin + H2O Cryptopygus antarcticus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Cryptopygus antarcticus C1IE32 gene CaLam
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Cryptopygus antarcticus ?
-
 ?
3.2.1.39 laminarin + H2O CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products Cryptopygus antarcticus D-glucose + laminaribiose + laminaritriose
-
 ?
3.2.1.39 laminarioligosaccharide + H2O CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products Cryptopygus antarcticus D-glucose + laminaribiose + laminaritriose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE Cryptopygus antarcticus
3.2.1.39 More the enzyme contains a putative signal peptide and a typical motif of glycosyl hydrolase family 16, EIDITE, CaLam homology modelling, overview Cryptopygus antarcticus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-beta-1,3-glucanase
-
 Cryptopygus antarcticus
3.2.1.39 More the enzyme belongs to the glycosyl hydrolase family 16, GH16 Cryptopygus antarcticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 50
-
-
 Cryptopygus antarcticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 6
-
-
 Cryptopygus antarcticus