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Literature summary extracted from
- Enzymatic degradation of beta- and mixed alpha,beta-oligopeptides (2006), Chem. Biodivers., 3, 1325-1348.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.13.20 | expression in Escherichia coli | Brucella anthropi |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.13.20 | active-site modeling of DmpA. Based on the crystal structure and the catalytic mechanism proposed for DmpA, carnosine is manually introduced into the active site of the enzyme. The model shows that carnosine fits excellently, in line with the high specific activity for this substrate | Brucella anthropi |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.25 | 0.7 | - |
beta-homoAla-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica |  |
| 3.4.11.25 | 0.76 | - |
beta-homoAla-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 1.23 | - |
beta-homoPhe-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica | |
| 3.4.11.25 | 1.45 | - |
beta-homoPhe-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 4.4 | - |
beta-Ala-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 8.1 | - |
beta-Ala-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica | |
| 3.4.13.20 | 0.0039 | - |
beta-Ala-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
| 3.4.13.20 | 0.019 | - |
beta-homoAla-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
| 3.4.13.20 | 1.1 | - |
beta-homoPhe-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.25 | Sphingosinicella microcystinivorans | A0MTQ2 | - |
- |
| 3.4.11.25 | Sphingosinicella microcystinivorans Y2 | A0MTQ2 | - |
- |
| 3.4.11.25 | Sphingosinicella xenopeptidilytica | Q52VH2 | - |
- |
| 3.4.11.25 | Sphingosinicella xenopeptidilytica 3-2W4 | Q52VH2 | - |
- |
| 3.4.13.20 | Brucella anthropi | Q59632 | - |
- |
| 3.4.13.20 | Brucella anthropi LMG7991 | Q59632 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.25 | - |
Sphingosinicella xenopeptidilytica |
| 3.4.11.25 | - |
Sphingosinicella microcystinivorans |
| 3.4.13.20 | - |
Brucella anthropi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.25 | (beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O | completely degraded to the corresponding beta-homoamino acids within 312 h | Sphingosinicella xenopeptidilytica | beta-homoAla + beta-homoLys + beta-homoPhe | - |
? | |
| 3.4.11.25 | (beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O | completely degraded to the corresponding beta-homoamino acids within 312 h | Sphingosinicella microcystinivorans | beta-homoAla + beta-homoLys + beta-homoPhe | - |
? | |
| 3.4.11.25 | (beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O | completely degraded to the corresponding beta-homoamino acids within 312 h | Sphingosinicella xenopeptidilytica 3-2W4 | beta-homoAla + beta-homoLys + beta-homoPhe | - |
? | |
| 3.4.11.25 | (beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O | completely degraded to the corresponding beta-homoamino acids within 312 h | Sphingosinicella microcystinivorans Y2 | beta-homoAla + beta-homoLys + beta-homoPhe | - |
? | |
| 3.4.11.25 | beta-Ala-Ile-beta-homoTyr + H2O | hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-Ala + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-Ala-Ile-beta-homoTyr + H2O | hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-Ala + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-Ala-Ile-beta-homoTyr + H2O | hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica 3-2W4 | beta-Ala + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-Ala-Ile-beta-homoTyr + H2O | hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans Y2 | beta-Ala + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-Ala-L-His + H2O | i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-Ala + L-His | - |
? | |
| 3.4.11.25 | beta-Ala-L-His + H2O | i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-Ala + L-His | - |
? | |
| 3.4.11.25 | beta-Ala-L-His + H2O | i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica 3-2W4 | beta-Ala + L-His | - |
? | |
| 3.4.11.25 | beta-Ala-L-His + H2O | i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans Y2 | beta-Ala + L-His | - |
? | |
| 3.4.11.25 | beta-Ala-p-nitroanilide + H2O | - |
Sphingosinicella xenopeptidilytica | beta-Ala + p-nitroaniline | - |
? | |
| 3.4.11.25 | beta-Ala-p-nitroanilide + H2O | - |
Sphingosinicella microcystinivorans | beta-Ala + p-nitroaniline | - |
? | |
| 3.4.11.25 | beta-Ala-p-nitroanilide + H2O | - |
Sphingosinicella xenopeptidilytica 3-2W4 | beta-Ala + p-nitroaniline | - |
? | |
| 3.4.11.25 | beta-Ala-p-nitroanilide + H2O | - |
Sphingosinicella microcystinivorans Y2 | beta-Ala + p-nitroaniline | - |
? | |
| 3.4.11.25 | beta-homoAla-beta-homoLeu + H2O | preferred substrate | Sphingosinicella microcystinivorans | beta-homoAla + beta-homoLeu | - |
? | |
| 3.4.11.25 | beta-homoAla-beta-homoLeu + H2O | hydrolysis at 31% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoAla + beta-homoLeu | - |
? | |
| 3.4.11.25 | beta-homoAla-p-nitroanilide + H2O | - |
Sphingosinicella xenopeptidilytica | beta-homoAla + p-nitroaniline | - |
? | |
| 3.4.11.25 | beta-homoAla-p-nitroanilide + H2O | - |
Sphingosinicella microcystinivorans | beta-homoAla + p-nitroaniline | - |
? | |
| 3.4.11.25 | beta-homoArg-Ile-beta-homoTyr + H2O | hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoArg + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoArg-Ile-beta-homoTyr + H2O | hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoArg + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoGln-Ile-beta-homoTyr + H2O | hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoGln + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoGln-Ile-beta-homoTyr + H2O | hydrolysis at 0.25% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoGln + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoHis-Ile-beta-homoTyr + H2O | hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoHis + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoHis-Ile-beta-homoTyr + H2O | hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoHis + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoLeu-Ile-beta-homoTyr + H2O | hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoLeu + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoLeu-Ile-beta-homoTyr + H2O | hydrolysis at 54% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoLeu + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoLys-Ile-beta-homoTyr + H2O | hydrolysis at 0.48% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoLys + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoLys-Ile-beta-homoTyr + H2O | hydrolysis at 0.5% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoLys + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoPhe-Ile-beta-homoTyr + H2O | hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoPhe + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoPhe-Ile-beta-homoTyr + H2O | hydrolysis at 19% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoPhe + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoPhe-p-nitroanilide + H2O | - |
Sphingosinicella xenopeptidilytica | beta-homoPhe + p-nitroaniline | - |
? | |
| 3.4.11.25 | beta-homoPhe-p-nitroanilide + H2O | - |
Sphingosinicella microcystinivorans | beta-homoPhe + p-nitroaniline | - |
? | |
| 3.4.11.25 | beta-homoSer-Ile-beta-homoTyr + H2O | hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoSer + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoSer-Ile-beta-homoTyr + H2O | hydrolysis at 2.7% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoSer + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoThr-Ile-beta-homoTyr + H2O | hydrolysis at 1.6% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoThr + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoThr-Ile-beta-homoTyr + H2O | hydrolysis at 1.9% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoThr + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoTrp-Ile-beta-homoTyr + H2O | hydrolysis at 1.2% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoTrp + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoTrp-Ile-beta-homoTyr + H2O | hydrolysis at 1.3% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoTrp + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoTyr-Ile-beta-homoTyr + H2O | hydrolysis at 13% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoTyr + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoTyr-Ile-beta-homoTyr + H2O | hydrolysis at 7% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoTyr + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoVal-beta-homoAla-beta-homoLeu + H2O | hydrolysis at 27% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoVal + beta-homoAla-beta-homoLeu | - |
? | |
| 3.4.11.25 | beta-homoVal-beta-homoAla-beta-homoLeu + H2O | hydrolysis at 89% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoVal + beta-homoAla-beta-homoLeu | - |
? | |
| 3.4.11.25 | beta-homoVal-beta-homoArg-beta-homoArg + H2O | hydrolysis at 0.2% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoVal + beta-homoArg-beta-homoArg | - |
? | |
| 3.4.11.25 | beta-homoVal-beta-homoArg-beta-homoArg + H2O | hydrolysis at 1.4% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoVal + beta-homoArg-beta-homoArg | - |
? | |
| 3.4.11.25 | beta-homoVal-beta-homoIle-beta-homoTyr + H2O | completely hydrolyzed. Hydrolysis at 15% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoVal + beta-homoIle + beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoVal-beta-homoIle-beta-homoTyr + H2O | completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoVal + beta-homoIle + beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoVal-beta-homoIle-Tyr + H2O | completely hydrolyzed. Hydrolysis at 20% compared to hydrolysis of beta-(3-homoVal)-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoVal + beta-homoIle + Tyr | - |
? | |
| 3.4.11.25 | beta-homoVal-beta-homoIle-Tyr + H2O | completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoVal + beta-homoIle + Tyr | - |
? | |
| 3.4.11.25 | beta-homoVal-Ile-beta-homoTyr + H2O | only the N-terminal beta-homoVal is released. Hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoVal + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoVal-Ile-beta-homoTyr + H2O | only the N-terminal beta-homoVal is released. Hydrolysis at 28% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | beta-homoVal + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | beta-homoVal-Ile-Tyr + H2O | only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | beta-homoVal + Ile-Tyr | - |
? | |
| 3.4.11.25 | D-beta-homoVal-Ile-beta-homoTyr + H2O | hydrolysis at 0.5% compared to hydrolysis of beta-homoAla-beta-homoLeu | Sphingosinicella microcystinivorans | D-beta-homoVal + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | D-beta-homoVal-Ile-beta-homoTyr + H2O | hydrolysis at 0.8% compared to hydrolysis of beta-homoVal-Ile-Tyr | Sphingosinicella xenopeptidilytica | D-beta-homoVal + Ile-beta-homoTyr | - |
? | |
| 3.4.11.25 | additional information | all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded | Sphingosinicella xenopeptidilytica | ? | - |
? | |
| 3.4.11.25 | additional information | all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded | Sphingosinicella microcystinivorans | ? | - |
? | |
| 3.4.11.25 | additional information | all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded | Sphingosinicella xenopeptidilytica 3-2W4 | ? | - |
? | |
| 3.4.11.25 | additional information | all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded | Sphingosinicella microcystinivorans Y2 | ? | - |
? | |
| 3.4.11.25 | Val-Ile-Tyr + H2O | only the N-terminal beta-homoVal is released. Preferred substrate | Sphingosinicella xenopeptidilytica | beta-homoVal + Ile-Tyr | - |
? | |
| 3.4.13.20 | (beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O | DmpA cleaves the N-terminal beta-homoAla, no further hydrolysis is observed within 13 days | Brucella anthropi | beta-homoAla + beta-homoLys-beta-homoPhe-beta-homoAla-beta-homoLys-beta-homoPhe | - |
? | |
| 3.4.13.20 | (beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O | DmpA cleaves the N-terminal beta-homoAla, no further hydrolysis is observed within 13 days | Brucella anthropi LMG7991 | beta-homoAla + beta-homoLys-beta-homoPhe-beta-homoAla-beta-homoLys-beta-homoPhe | - |
? | |
| 3.4.13.20 | beta-Ala-4-nitroanilide + H2O | - |
Brucella anthropi | beta-Ala + 4-nitroaniline | - |
? | |
| 3.4.13.20 | beta-Ala-4-nitroanilide + H2O | - |
Brucella anthropi LMG7991 | beta-Ala + 4-nitroaniline | - |
? | |
| 3.4.13.20 | beta-Ala-Ile-beta-homoTyr + H2O | hydrolysis at 1.3% compared to hydrolysis of carnosine | Brucella anthropi | beta-Ala + Ile-beta-homoTyr | - |
? | |
| 3.4.13.20 | beta-Ala-Ile-beta-homoTyr + H2O | hydrolysis at 1.3% compared to hydrolysis of carnosine | Brucella anthropi LMG7991 | beta-Ala + Ile-beta-homoTyr | - |
? | |
| 3.4.13.20 | beta-Ala-L-His + H2O | i.e. carnosine, preferred substrate | Brucella anthropi | beta-Ala + L-His | - |
? | |
| 3.4.13.20 | beta-Ala-L-His + H2O | i.e. carnosine, preferred substrate | Brucella anthropi LMG7991 | beta-Ala + L-His | - |
? | |
| 3.4.13.20 | beta-homoAla-4-nitroanilide + H2O | - |
Brucella anthropi | beta-homoAla + 4-nitroaniline | - |
? | |
| 3.4.13.20 | beta-homoAla-beta-homoLeu + H2O | hydrolysis at 55% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoAla + beta-homoLeu | - |
? | |
| 3.4.13.20 | beta-homoLeu-Ile-beta-homoTyr + H2O | hydrolysis at 0.01% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoLeu + Ile-beta-homoTyr | - |
? | |
| 3.4.13.20 | beta-homoPhe-4-nitroanilide + H2O | - |
Brucella anthropi | beta-homoPhe + 4-nitroaniline | - |
? | |
| 3.4.13.20 | beta-homoSer-Ile-beta-homoTyr + H2O | hydrolysis at 0.06% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoSer + Ile-beta-homoTyr | - |
? | |
| 3.4.13.20 | beta-homoVal-beta-homoAla-beta-homoLeu + H2O | hydrolysis at 0.19% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoVal + beta-homoAla-beta-homoLeu | - |
? | |
| 3.4.13.20 | beta-homoVal-Ile-beta-homoTyr + H2O | hydrolysis at 0.01% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoVal + Ile-beta-homoTyr | - |
? | |
| 3.4.13.20 | beta-homoVal-Ile-Tyr + H2O | hydrolysis at 0.09% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoVal + Ile-Tyr | - |
? | |
| 3.4.13.20 | additional information | cleaves beta- and mixed alpha,beta-peptides and amides, but a short side chain of the N-terminal beta-amino acid residue seems to be a prerequisite, since only peptides carrying N-terminal betahGly and betahAla are hydrolyzed with good efficiencies. Tripeptides carrying two consecutive N-terminal beta-homoamino acids as well as peptides with alpha-amino acids in the N-terminal position do not serve as substrates for DmpA | Brucella anthropi | ? | - |
? | |
| 3.4.13.20 | additional information | cleaves beta- and mixed alpha,beta-peptides and amides, but a short side chain of the N-terminal beta-amino acid residue seems to be a prerequisite, since only peptides carrying N-terminal betahGly and betahAla are hydrolyzed with good efficiencies. Tripeptides carrying two consecutive N-terminal beta-homoamino acids as well as peptides with alpha-amino acids in the N-terminal position do not serve as substrates for DmpA | Brucella anthropi LMG7991 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.25 | 3-2W4 BapA | - |
Sphingosinicella xenopeptidilytica |
| 3.4.11.25 | Y2 BapA | - |
Sphingosinicella microcystinivorans |
| 3.4.13.20 | DmpA | - |
Brucella anthropi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.25 | 25 | - |
assay at | Sphingosinicella xenopeptidilytica |
| 3.4.11.25 | 25 | - |
assay at | Sphingosinicella microcystinivorans |
| 3.4.13.20 | 25 | - |
assay at | Brucella anthropi |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.25 | 0.13 | - |
beta-Ala-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 0.22 | - |
beta-homoPhe-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 0.75 | - |
beta-Ala-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica | |
| 3.4.11.25 | 2.89 | - |
beta-homoAla-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 13.2 | - |
beta-homoAla-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica | |
| 3.4.11.25 | 30.2 | - |
beta-homoPhe-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica | |
| 3.4.13.20 | 0.03 | - |
beta-homoPhe-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
| 3.4.13.20 | 30.5 | - |
beta-Ala-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
| 3.4.13.20 | 51.4 | - |
beta-homoAla-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.25 | 8 | - |
assay at | Sphingosinicella xenopeptidilytica |
| 3.4.11.25 | 8 | - |
assay at | Sphingosinicella microcystinivorans |
| 3.4.13.20 | 8 | - |
assay at | Brucella anthropi |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.25 | 0.029 | - |
beta-Ala-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 0.092 | - |
beta-Ala-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica | |
| 3.4.11.25 | 0.154 | - |
beta-homoPhe-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 3.8 | - |
beta-homoAla-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella microcystinivorans | |
| 3.4.11.25 | 18.7 | - |
beta-homoAla-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica | |
| 3.4.11.25 | 24.6 | - |
beta-homoPhe-p-nitroanilide | pH 8.0, 25°C | Sphingosinicella xenopeptidilytica | |
| 3.4.13.20 | 0.027 | - |
beta-homoPhe-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
| 3.4.13.20 | 2600 | - |
beta-homoAla-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
| 3.4.13.20 | 7800 | - |
beta-Ala-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
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Release 2023.1 (January 2023)
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