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Literature summary extracted from

  • Adrangi, S.; Faramarzi, M.A.; Shahverdi, A.R.; Sepehrizadeh, Z.
    Purification and characterization of two extracellular endochitinases from Massilia timonae (2010), Carbohydr. Res., 345, 402-407.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 EDTA 1 mM, 64% of initial activity, isoform Chi-56, 65% of initial activity, isoform Chi-64 Massilia timonae
3.2.1.14 Hg2+ 1 mM, 58% of initial activity, isoform Chi-56, 54% of initial activity, isoform Chi-64 Massilia timonae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Mn2+ 1 mM, 117% of initial activity, isoform Chi-56, 131% of initial activity, isoform Chi-64 Massilia timonae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 28000
-
 gel filtration, isoform Chi-64 Massilia timonae
3.2.1.14 56000
-
 gel filtration, isoform Chi-56 Massilia timonae
3.2.1.14 64000
-
 x * 64000, isoform Chi-64, SDS-PAGE Massilia timonae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Massilia timonae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O isolated from crab shell, 18% of the activity with colloidal chitin for isoform Chi-56, 27% for isoform Chi-64 Massilia timonae ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Massilia timonae ?
-
 ?
3.2.1.14 additional information no substrate: flake chitin, chitosan with degree of acetylation 96.9, carboxymethyl cellulose. No exochitinase activity for both isoform Chi-56 and Chi-64 Massilia timonae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 64000, isoform Chi-64, SDS-PAGE Massilia timonae
3.2.1.14 monomer 1 * 56000, isoform Chi-560, SDS-PAGE Massilia timonae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 55
-
 isoform Chi-56 Massilia timonae
3.2.1.14 60
-
 isoform Chi-64 Massilia timonae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
 both isoform Chi-56 and Chi-64 Massilia timonae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Massilia timonae isoelectric focusing, both isoform Chi-56 and Chi-64
-
 8.5