EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.73 | gene LicB, expression of mature enzyme and isolated catalytic domain, as well as of the enzyme truncation mutants in Escherichia coli strain BL21 | Acetivibrio thermocellus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.73 | additional information | construction of diverse enzyme truncation mutants for domain functional analysis, overview | Acetivibrio thermocellus |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.73 | Ca2+ stabilizes the enzyme at higher temperatures | Acetivibrio thermocellus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.73 | additional information | Ca2+ stabilizes the enzyme at higher temperatures | Acetivibrio thermocellus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.73 | additional information | Acetivibrio thermocellus | lichenase hydrolyses only beta-1,4-linkages that are adjacent to beta-1,3-linkages in beta-glucans, mainly producing cellobiosyltriose and cellotriosyltetraose | ? | - |
? | |
3.2.1.73 | additional information | Acetivibrio thermocellus ZJL4 | lichenase hydrolyses only beta-1,4-linkages that are adjacent to beta-1,3-linkages in beta-glucans, mainly producing cellobiosyltriose and cellotriosyltetraose | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.73 | Acetivibrio thermocellus | - |
gene LicB | - |
3.2.1.73 | Acetivibrio thermocellus ZJL4 | - |
gene LicB | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.73 | lichenin + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
3.2.1.73 | lichenin + H2O | - |
Acetivibrio thermocellus ZJL4 | ? | - |
? | |
3.2.1.73 | additional information | lichenase hydrolyses only beta-1,4-linkages that are adjacent to beta-1,3-linkages in beta-glucans, mainly producing cellobiosyltriose and cellotriosyltetraose | Acetivibrio thermocellus | ? | - |
? | |
3.2.1.73 | additional information | lichenase hydrolyses only beta-1,4-linkages that are adjacent to beta-1,3-linkages in beta-glucans, mainly producing cellobiosyltriose and cellotriosyltetraose | Acetivibrio thermocellus ZJL4 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.73 | More | the Thr-Pro box has a positive effect while the dockerin domain has a negative impact on the properties of LicMB. The N-terminal residues 10-25 and C-terminal residues 1-9 of LicMB-CD are necessary to retain high thermostability, while the N-terminal residues 1-7 and C-terminal residues 1-3 are not necessary to maintain enzymatic activity | Acetivibrio thermocellus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.73 | 1,3-1,4-beta-D-glucanase | - |
Acetivibrio thermocellus |
3.2.1.73 | Lichenase | - |
Acetivibrio thermocellus |
3.2.1.73 | LicMB | - |
Acetivibrio thermocellus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | additional information | - |
Ca2+ stabilizes the enzyme at higher temperatures, thermal stability of recombinant wild-type enzyme and mutants in presence and absence of Ca2+, overview | Acetivibrio thermocellus |
3.2.1.73 | 75 | - |
purified recombinant wild-type enzyme, 10 min, 51% remaining activity in absence of Ca2+, 86% in presence of 1 mM Ca2+ | Acetivibrio thermocellus |
3.2.1.73 | 80 | - |
purified recombinant wild-type enzyme, 5 min, 54% remaining activity in absence of Ca2+, 73% in presence of 1 mM Ca2+ | Acetivibrio thermocellus |