EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.26.5 | pET-15b-derived plasmid endcoding the C5 protein expressed in Escherichia coli BL21(DE3). Into pUC19 vector and transformed into Escherichia coli strain JM109 | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.5 | Mg2+ | required for activation | Escherichia coli | |
3.1.26.5 | Mg2+ | required for activation | Pyrococcus horikoshii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.5 | Escherichia coli | - |
- |
- |
3.1.26.5 | Pyrococcus horikoshii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.5 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.5 | additional information | is unable to activate non-cognate RNase P RNAs, Pyrococcus horikoshii RNase P RNA and Escherichia coli RNase P RNA. Chimeric RNase P RNAs composed of the Escherichia coli RNA C-domain and Pyrococcus horikoshii S-domain or composed of the Pyrococcus horikoshii C-domain and Escherichia coli RNA S-domain, respectively, exhibit activity. C5 protein is involved in activation of the Escherichia coli pRNA C-domain | Escherichia coli | ? | - |
? | |
3.1.26.5 | additional information | RNase P proteins Pop5, Rpp21, Rpp29, Rpp30 and Rpp38 are unable to activate non-cognate RNase P RNAs, Pyrococcus horikoshii RNase P RNA and Escherichia coli RNase P RNA. Chimeric RNase P RNAs composed of the Escherichia coli RNA C-domain and Pyrococcus horikoshii S-domain or composed of the Pyrococcus horikoshii C-domain and Escherichia coli RNA S-domain, respectively, exhibit activity. Pop5 and Rpp30 are involved in activation of the Pyrococcus horikoshii pRNA C-domain, whereas Rpp21 and Rpp29 are implicated in stabilization of the Pyrococcus horikoshii pRNA S-domain | Pyrococcus horikoshii | ? | - |
? | |
3.1.26.5 | pre-tRNATyr + H2O | cleaves efficiently at a single phosphodieste bond between positions U59 and C60 | Escherichia coli | mature tRNATyr + 5'-oligoribonucleotide | - |
? | |
3.1.26.5 | pre-tRNATyr + H2O | is completely processed. Cleaves efficiently at a single phosphodieste bond between positions U59 and C60 | Pyrococcus horikoshii | mature tRNATyr + 5'-oligoribonucleotide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.5 | Pop5 | - |
Pyrococcus horikoshii |
3.1.26.5 | Protein C5 | - |
Escherichia coli |
3.1.26.5 | ribonuclease P | - |
Escherichia coli |
3.1.26.5 | ribonuclease P | - |
Pyrococcus horikoshii |
3.1.26.5 | RNase P | - |
Escherichia coli |
3.1.26.5 | RNase P | - |
Pyrococcus horikoshii |
3.1.26.5 | Rpp21 | - |
Pyrococcus horikoshii |
3.1.26.5 | Rpp29 | - |
Pyrococcus horikoshii |
3.1.26.5 | Rpp30 | - |
Pyrococcus horikoshii |
3.1.26.5 | Rpp38 | - |
Pyrococcus horikoshii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 50 | - |
- |
Escherichia coli |
3.1.26.5 | 50 | - |
- |
Pyrococcus horikoshii |