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Literature summary extracted from

  • Gao, D.; Chundawat, S.P.; Krishnan, C.; Balan, V.; Dale, B.E.
    Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover (2010), Biores. Technol., 101, 2770-2781.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Trichoderma reesei P07987
-
-
3.2.1.91 Trichoderma reesei P62694
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 native enzyme from commercial enzyme blend, Spezyme CP by ammonium sulfate fractionation, anion exchange chromatography, and p-aminophenyl-1-thio-beta-D-cellobioside-based affinity chromatography Trichoderma reesei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.91 commercial preparation commercial enzyme blend, Spezyme CP Trichoderma reesei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma reesei 4-nitrophenol + cellobiose
-
 ?
3.2.1.91 avicel + H2O microcrystalline cellulose Trichoderma reesei ?
-
 ?
3.2.1.91 additional information no activity on oat spelt xylan, carboxymethylcellulose, xylobiose, cellobiose, 4-nitrophenyl-cellobiose, and 4-nitrophenyl derivatives of other disaccharides, overview Trichoderma reesei ?
-
 ?
3.2.1.91 additional information no activity on oat spelt xylan, carboxymethylcellulose, xylobiose, cellobiose, and 4-nitrophenyl derivatives of other disaccharides, overview Trichoderma reesei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 More identification of enzyme peptides by mass spectrometry Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CBH I
-
 Trichoderma reesei
3.2.1.91 CBH II
-
 Trichoderma reesei
3.2.1.91 cellobiohydrolase I
-
 Trichoderma reesei
3.2.1.91 cellobiohydrolase II
-
 Trichoderma reesei
3.2.1.91 More the enzyme belongs to the glycosylhydrolase family GH6A Trichoderma reesei
3.2.1.91 More the enzyme belongs to the glycosylhydrolase family GH7A Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 50
-
 assay at Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 5
-
 assay at Trichoderma reesei