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Literature summary extracted from
- Purification and enzymatic characterization of two beta-endoxylanases from Trichoderma sp. K9301 and their actions in xylooligosaccharide production (2009), Biores. Technol., 100, 5230-5236.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.8 | 4.47 | - |
beta-1,4-xylan | pH 5.6, 50°C, isozyme EX1, beechwood xylan | Trichoderma sp. |  |
| 3.2.1.8 | 8.33 | - |
beta-1,4-xylan | pH 5.6, 50°C, isozyme EX2, beechwood xylan | Trichoderma sp. | |
| 3.2.1.8 | 8.87 | - |
beta-1,4-xylan | pH 5.6, 50°C, isozyme EX1, birchwood xylan | Trichoderma sp. | |
| 3.2.1.8 | 11.7 | - |
beta-1,4-xylan | pH 5.6, 50°C, isozyme EX2, birchwood xylan | Trichoderma sp. | |
| 3.2.1.8 | 20 | - |
beta-1,4-xylan | pH 5.6, 50°C, isozyme EX1, oat spelt xylan | Trichoderma sp. | |
| 3.2.1.8 | 22.2 | - |
beta-1,4-xylan | pH 5.6, 50°C, isozyme EX2, oat spelt xylan | Trichoderma sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.8 | extracellular | the endoxylanases are secreted | Trichoderma sp. | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 20100 | - |
x * 30100, isozyme EX1, SDS-PAGE, x * 20100, isozyme EX2, SDS-PAGE | Trichoderma sp. |
| 3.2.1.8 | 30100 | - |
x * 30100, isozyme EX1, SDS-PAGE, x * 20100, isozyme EX2, SDS-PAGE | Trichoderma sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.8 | Trichoderma sp. | - |
two acidic beta-endoxylanases EX1 and EX2 | - |
| 3.2.1.8 | Trichoderma sp. K9301 | - |
two acidic beta-endoxylanases EX1 and EX2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.8 | native isozymes EX1 and EX2 are purified from crude extract of the strain K9301 in solid fermentation by anion exchange chromatography and gel filtration to homogeneity | Trichoderma sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | additional information | - |
2836 U/g dry weight, secreted enzymes | Trichoderma sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.8 | beta-1,4-xylan + H2O | isozyme EX1 exhibits better affinity but lower hydrolytic efficiency than isozyme EX2 to xylans from beechwood, birchwood, and oat-spelt | Trichoderma sp. | xylo-oligosaccharides | product analysis by HPLC, overview | ? | |
| 3.2.1.8 | beta-1,4-xylan + H2O | isozyme EX1 exhibits better affinity but lower hydrolytic efficiency than isozyme EX2 to xylans from beechwood, birchwood, and oat-spelt | Trichoderma sp. K9301 | xylo-oligosaccharides | product analysis by HPLC, overview | ? | |
| 3.2.1.8 | xylo-oligosaccharide + H2O | substrates from corn cobs | Trichoderma sp. | ? | - |
? | |
| 3.2.1.8 | xylo-oligosaccharide + H2O | substrates from corn cobs | Trichoderma sp. K9301 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.8 | ? | x * 30100, isozyme EX1, SDS-PAGE, x * 20100, isozyme EX2, SDS-PAGE | Trichoderma sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.8 | beta-endoxylanase | - |
Trichoderma sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 55 | - |
isozyme EX2 | Trichoderma sp. |
| 3.2.1.8 | 70 | - |
isozyme EX1 | Trichoderma sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 30 | 70 | activity range, isozyme EX2 | Trichoderma sp. |
| 3.2.1.8 | 30 | 90 | activity range, isozyme EX1 | Trichoderma sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 40 | 60 | purified isozyme EX1, stable at temperatures below 40°C, retains 50% activity after incubation at 50°C for 30 h, but it loses its activity rapidly at 60°C for 1 h | Trichoderma sp. |
| 3.2.1.8 | 50 | 60 | purified EX2, 48 h, 50% activity remaining | Trichoderma sp. |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.8 | additional information | - |
additional information | - |
Trichoderma sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 5 | - |
isozyme EX2 | Trichoderma sp. |
| 3.2.1.8 | 5.5 | - |
isozyme EX1 | Trichoderma sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 4 | 9 | activity range of EX1 and EX2, profiles, overview | Trichoderma sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.8 | 3 | 10 | stability profile of EX1 and EX2, highly to completely stable at pH 4.0 to pH 9.0, inactivation at pH 3.0 and pH 10.0, overview | Trichoderma sp. |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.8 | additional information | - |
additional information | - |
Trichoderma sp. |
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