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Literature summary extracted from

  • Kudoh, T.; Park, C.S.; Lefurgy, S.T.; Sun, M.; Michels, T.; Leyh, T.S.; Silverman, R.B.
    Mevalonate analogues as substrates of enzymes in the isoprenoid biosynthetic pathway of Streptococcus pneumoniae (2009), Bioorg. Med. Chem., 18, 1124-1134.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 overexpression in Escherichia coli as dual-tagged His6-GST fusion protein Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.36 diphosphomevalonate strong feedback inhibitor of the enzyme Streptococcus pneumoniae
2.7.1.36 additional information synthesis of a series of C3-substituted mevalonate analogues to probe the steric and electronic requirements of the mevalonate kinase active site for development of specific mechanism-based inhibitors, overview Streptococcus pneumoniae
2.7.4.2 additional information synthesis of a series of C3-substituted mevalonate analogues to probe the steric and electronic requirements off the phosphomevalonate kinase active site for development of specific mechanism-based inhibitors, overview Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.2 Mg2+
-
 Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate Streptococcus pneumoniae
-
 ADP + (R)-5-phosphomevalonate
-
 ?
2.7.4.2 ATP + (R)-5-phosphomevalonate Streptococcus pneumoniae
-
 ADP + (R)-5-diphosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Streptococcus pneumoniae Q8DR51
-
-
2.7.4.2 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 recombinant dual-tagged His6-GST enzyme from Escherichia coli by affinity chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Streptococcus pneumoniae ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 additional information the recombinant enzyme phosphorylates racemic vinyl, ethyl, n-propyl, n-butyl, i-butyl, 2-propenyl, allyl, ethynyl, and 1-propynyl mevalonate lactone analogues, that are diphosphorylated by phosphomevalonate kinase, EC 2.7.4.2, in a second step, overview Streptococcus pneumoniae ?
-
 ?
2.7.4.2 ATP + (R)-5-phosphomevalonate
-
 Streptococcus pneumoniae ADP + (R)-5-diphosphomevalonate
-
 ?
2.7.4.2 additional information the recombinant enzyme phosphorylates racemic vinyl, ethyl, n-propyl, n-butyl, i-butyl, 2-propenyl, allyl, ethynyl, and 1-propynyl mevalonate lactone analogues, that are phosphorylated by mevalonate kinase, EC 2.7.1.36, in a first step, overview Streptococcus pneumoniae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.2 PMK
-
 Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.36 ATP
-
 Streptococcus pneumoniae
2.7.4.2 ATP
-
 Streptococcus pneumoniae

General Information

EC Number General Information Comment Organism
2.7.1.36 metabolism the enzyme is regulating the mevalonate pathway in Streptococcus pneumoniae, it is strongly feedback-inhibited by diphosphomevalonate, the penultimate compound in the pathway, overview Streptococcus pneumoniae