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Literature summary extracted from

  • Hormann, K; Andreesen, J.R.
    Purification and characterization of a pyrrole-2-carboxylate oxygenase from Arthrobacter strain Py (1994), Biol. Chem. Hoppe-Seyler, 375, 211-218.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.130 FAD and dithioerythritol stabilize enzyme activity during purification Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.130 5,5'-dithiobis(2-nitrobenzoic acid) 0.5 mM, 87% loss of activity Arthrobacter sp.
1.14.13.130 Cu+ 0.001-0.01 mM, 93% loss of activity Arthrobacter sp.
1.14.13.130 Hg2+ 0.01-0.1 mM, 96% loss of activity Arthrobacter sp.
1.14.13.130 additional information no inhibition by 1 mM EDTA or 1 mM arsenite Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.130 60000
-
 x * 60000, SDS-PAGE Arthrobacter sp.
1.14.13.130 160000
-
 gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 Arthrobacter sp. the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
 ?
1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 Arthrobacter sp. Py1 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.130 Arthrobacter sp.
-
-
-
1.14.13.130 Arthrobacter sp. Py1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.130 FAD and dithioerythritol stabilize enzyme activity during purification Arthrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.130 culture condition:pyrrole-2-carboxylate-grown cell
-
 Arthrobacter sp.
-

Storage Stability

EC Number Storage Stability Organism
1.14.13.130 -20°C, several months, stable Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Arthrobacter sp. 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
 ?
1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate Arthrobacter sp. 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
 ?
1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Arthrobacter sp. Py1 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
 ?
1.14.13.130 pyrrole-2-carboxylate + NADH + H+ + O2 no activity with NADPH. Narrow substrate specificity. Besides pyrrole-2-carboxylate, only pyrrole, pyrrole-2-aldehyde, and indole-2-carboxylate stimulate the oxygen consumption at a very low rate Arthrobacter sp. Py1 5-hydroxypyrrole-2-carboxylate + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.130 ? x * 60000, SDS-PAGE Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.130 7.5 9
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.130 7.2 9.5 pH 7.2: 65% of maximal activity, pH 9.5: 68% of maximal activity Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.130 FAD flavoprotein. Contains 2.7-3.6 mol of FAD per mol of enzyme Arthrobacter sp.
1.14.13.130 NADH no activity with NADPH Arthrobacter sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.13.130 Arthrobacter sp.
-
-
 4.6

Expression

EC Number Organism Comment Expression
1.14.13.130 Arthrobacter sp. activity is induced by wrowth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy up

General Information

EC Number General Information Comment Organism
1.14.13.130 physiological function the enzyme initiates the degradation of pyrrole-2-carboxylate. Growth on pyrrole-2-carboxylate as the sole source of carbon, nitrogen and energy Arthrobacter sp.