EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.4 | cDNA cloned into the pGEM-T vector and transformed into Escherichia coli strain DH5alpha | Bothrops asper |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.4 | 14180 | - |
sequence analysis | Bothrops asper |
3.1.1.4 | 14210 | - |
electrospray ionization | Bothrops asper |
3.1.1.4 | 15000 | - |
1 * 15000, SDS-PAGE, 1 * 16000, SDS-PAGE, both under reducing and non-reducing conditions | Bothrops asper |
3.1.1.4 | 16000 | - |
1 * 15000, SDS-PAGE, 1 * 16000, SDS-PAGE, both under reducing and non-reducing conditions | Bothrops asper |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.4 | Bothrops asper | P20474 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.4 | by gel filtration | Bothrops asper |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.4 | venom | - |
Bothrops asper | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.4 | egg yolk phospholipid + H2O | - |
Bothrops asper | ? | - |
? | |
3.1.1.4 | sn-3-phosphatidylcholine + H2O | - |
Bothrops asper | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.4 | monomer | 1 * 15000, SDS-PAGE, 1 * 16000, SDS-PAGE, both under reducing and non-reducing conditions | Bothrops asper |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.4 | phospholipase A2 | - |
Bothrops asper |
3.1.1.4 | PLA2 | - |
Bothrops asper |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.4 | Bothrops asper | 2D electrophoresis | - |
4.9 |
3.1.1.4 | Bothrops asper | sequence analysis | - |
5.05 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.1.4 | physiological function | acidic PLA2-II, in contrast to the basic Asp49 counterparts present in the same venom, lacks myotoxic, cytotoxic, and anticoagulant activities. It does not show hypotensive and anti-platelet aggregation activities and is not lethal to mice at intravenous doses up to 100 microg (5.9 microg/g), indicating its lack of neurotoxic activity. It shows a moderate induction of local edema in vivo. Acidic PLA2-II does not play a key role in the pathophysiology of envenomings, its purpose may be restricted to digestive functions. Acidic and basic PLA2s form two different antigenic groups in venom | Bothrops asper |