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Literature summary extracted from

  • Sherratt, A.R.; Braganza, M.V.; Nguyen, E.; Ducat, T.; Goto, N.K.
    Insights into the effect of detergents on the full-length rhomboid protease from Pseudomonas aeruginosa and its cytosolic domain (2009), Biochim. Biophys. Acta, 1788, 2444-2453.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.105 expressed as a C-terminal His-tagged fusion protein in Escherichia coli BL21 Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.105 1,2-dihexanoyl-sn-glycero-3-phosphocholine 1,2-dimyristoyl-sn-glycero-3-phosphocholine additionally added, paGlpG purified in detergent causes 37% reduction in activity Pseudomonas aeruginosa
3.4.21.105 1,2-dimyristoyl-sn-glycero-3-phosphocholine paGlpG purified in detergent causes 5% reduction in activity Pseudomonas aeruginosa
3.4.21.105 1-myristoyl-sn-glycero-3-phosphocholine paGlpG purified in detergent causes 10% reduction in activity Pseudomonas aeruginosa
3.4.21.105 1-palmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) paGlpG purified in detergent causes 20% reduction in activity Pseudomonas aeruginosa
3.4.21.105 3-[(3-cholamidopropyl)-dimethylammonio]-1-propansulfonate 1,2-dimyristoyl-sn-glycero-3-phosphocholine additionally added, paGlpG purified in detergent causes 37% reduction in activity Pseudomonas aeruginosa
3.4.21.105 dodecyl maltoside paGlpG purified in detergent causes 77% reduction in activity Pseudomonas aeruginosa
3.4.21.105 n-nonyl-beta-D-glucoside paGlpG purified in detergent causes 45% reduction in activity Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.105 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.105 rhomboid from Pseudomonas aeruginosa is purified in a range of membrane-mimetic media, its functional status in vitro and the NMR spectroscopic properties of these samples are investigated. NMR signals can only be observed from the cytosolic domain, and only in detergents that do not support rhomboid activity. In contrast, media that support rhomboid function do not show these resonances, suggesting an association between the cytosolic domain and the protein-detergent complex Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.105 beta-lactamase-Spitz transmembrane segment-maltose binding protein + H2O a fusion protein containing the Spitz TM segment fused to globular proteins at the N- and C-termini (beta-lactamase and maltose binding protein, respectively) Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.105 paGlpG
-
 Pseudomonas aeruginosa
3.4.21.105 Rhomboid
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
3.4.21.105 physiological function the cytosolic domain does not interact with the lipid membrane, but instead enhances rhomboid activity through interactions with some other part of the rhomboid, such as the catalytic core domain Pseudomonas aeruginosa