Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ito, T.; Sadamoto, R.; Naruchi, K.; Togame, H.; Takemoto, H.; Kondo, H.; Nishimura, S.
    Highly oriented recombinant glycosyltransferases: site-specific immobilization of unstable membrane proteins by using Staphylococcus aureus sortase A (2010), Biochemistry, 49, 2604-2614.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.70 17800
-
 SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 HisTrapHP column chromatography, gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 AHLPKTGLR + 5-aminopentan-1-ol
-
 Staphylococcus aureus ?
-
 ?
3.4.22.70 AHLPKTGLR + 6-aminohexyl 4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Staphylococcus aureus ?
-
 ?
3.4.22.70 AHLPKTGLR + N-(2-(2-ethoxyethoxy)ethanamine)biotin amide
-
 Staphylococcus aureus ?
-
 ?
3.4.22.70 AHLPKTGLR + N-hexylbiotin amide
-
 Staphylococcus aureus ?
-
 ?
3.4.22.70 AHLPKTGLR-NH2 + triglycine
-
 Staphylococcus aureus ?
-
 ?
3.4.22.70 additional information the enzyme cleaves the LPXTG sequence at the amide bond between the threonine and the glycine to form an acyl-enzyme complex. Nucleophilic attack by the amino group of the tri-glycine on the intermediate results in the formation of an LPXT-GGG bond and the liberation of the free enzyme Staphylococcus aureus ?
-
 ?
3.4.22.70 recombinant Helicobacter pylori R1,3-fucosyltransferase + triglycine
-
 Staphylococcus aureus ?
-
 ?
3.4.22.70 recombinant human beta1,4-galactosyltransferase + N-(2-(2-ethoxyethoxy)ethanamine)biotin amide
-
 Staphylococcus aureus ?
-
 ?
3.4.22.70 recombinant human beta1,4-galactosyltransferase + N-hexylbiotin amide
-
 Staphylococcus aureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.70 SrtA
-
 Staphylococcus aureus