EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.2.20 | expressed in Escherichia coli BL21(DE3) | Haemophilus influenzae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.2.20 | hanging drop vapor diffusion method | Haemophilus influenzae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.2.20 | D44A | putative catalytic residue | Haemophilus influenzae |
3.1.2.20 | N29A | involved in substrate binding | Haemophilus influenzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.20 | 0.0042 | - |
lauroyl-CoA | N29A mutant protein | Haemophilus influenzae | |
3.1.2.20 | 0.0066 | - |
lauroyl-CoA | wild-type protein | Haemophilus influenzae | |
3.1.2.20 | 0.013 | - |
isobutyryl-CoA | wild-type protein | Haemophilus influenzae | |
3.1.2.20 | 0.092 | - |
isobutyryl-CoA | N29A mutant protein | Haemophilus influenzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.2.20 | 17000 | - |
6 * 17000, crystal structure analysis | Haemophilus influenzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.20 | Haemophilus influenzae | P44886 | stain Rd KW20 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.20 | - |
Haemophilus influenzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.20 | butyryl-CoA + H2O | - |
Haemophilus influenzae | butanoate + CoA | - |
? | |
3.1.2.20 | butyrylpanthetheine phosphate + H2O | - |
Haemophilus influenzae | pantetheine phosphate + butyrate | - |
? | |
3.1.2.20 | isobutyryl-CoA + H2O | - |
Haemophilus influenzae | CoA + isobutyrate | - |
? | |
3.1.2.20 | lauroyl-CoA + H2O | - |
Haemophilus influenzae | CoA + lauroate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.2.20 | homohexamer | 6 * 17000, crystal structure analysis | Haemophilus influenzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.2.20 | acyl-Coenzyme A thioesterase | - |
Haemophilus influenzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.20 | additional information | - |
additional information | D44A mutant protein: value below 0.001 s-1 for isobutyryl-CoA and lauroyl-CoA | Haemophilus influenzae | |
3.1.2.20 | 0.08 | - |
butyrylpantetheine phosphate | wild-type protein | Haemophilus influenzae | |
3.1.2.20 | 2.34 | - |
isobutyryl-CoA | N29A mutant protein | Haemophilus influenzae | |
3.1.2.20 | 2.57 | - |
lauroyl-CoA | N29A mutant protein | Haemophilus influenzae | |
3.1.2.20 | 6.7 | - |
butyryl-CoA | wild-type protein | Haemophilus influenzae | |
3.1.2.20 | 9.2 | - |
lauroyl-CoA | wild-type protein | Haemophilus influenzae | |
3.1.2.20 | 138 | - |
isobutyryl-CoA | wild-type protein | Haemophilus influenzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.20 | 0.1 | - |
butyrylpantetheine phosphate | wild-type protein | Haemophilus influenzae | |
3.1.2.20 | 25 | - |
isobutyryl-CoA | N29A mutant protein | Haemophilus influenzae | |
3.1.2.20 | 100 | - |
butyryl-CoA | wild-type protein | Haemophilus influenzae | |
3.1.2.20 | 610 | - |
lauroyl-CoA | N29A mutant protein | Haemophilus influenzae | |
3.1.2.20 | 1400 | - |
lauroyl-CoA | wild-type protein | Haemophilus influenzae | |
3.1.2.20 | 11000 | - |
isobutyryl-CoA | wild-type protein | Haemophilus influenzae |