Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guo, R.-T.; Kuo, C.-J.; Ko, T.-P.; Chou, C.-C.; Liang, P.-H.; Wang, A.H.J.
    A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products (2004), Biochemistry, 43, 7678-7686.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.90 mutants A76Y, A76Y/S77F, F132A/L128A, F132A/L128A/I123A, and F132A/L128A/I123A/D62A to 3.1, 2.7, 3.3, 3.35 and 3.4 A resolution, respectively. Like wildtype OPPs, all mutant structures contain 12 alpha-helices, nine of them surrounding a large central cavity and an elongated tunnel-shaped active site cavity surrounded by four alpha-helices In the crystal structure of the A76Y/S77F mutant, F77 is pushed away by Y76, thereby creating more space between those two large amino acids to accommodate the C20 product. A large F132 residue at the bottom of the tunnel-shaped active site serves as the floor and determines the final product chain length. The substitution of F132 with a small Ala, thereby removing the blockade, leads to the synthesis of a C50 product larger than that produced by the wild-type enzyme Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.90 A76Y mutant produces only C20 cores instead of the C40 core of octaprenyl diphosphate Thermotoga maritima
2.5.1.90 A76Y/S77F mutant produces only C20 cores instead of the C40 core of octaprenyl diphosphate. The A76Y/S77F mutant synthesizes a larger amount of C20 than the A76Y mutant Thermotoga maritima
2.5.1.90 F132A steady-state activity 0.0051 per s. Product chain length C45 to C60 Thermotoga maritima
2.5.1.90 F132A/L128A steady-state activity 0.0008 per s. Product chain length C55, C60 Thermotoga maritima
2.5.1.90 F132A/L128A/I123A steady-state activity 0.00066 per s. Produuct chain length C55 to C75 Thermotoga maritima
2.5.1.90 F132A/L128A/I123A/D62A products reach C95, beyond the largest chain length generated by all known trans-prenyltransferases. Steady-state activity 0.00061 per s Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.90 Thermotoga maritima Q9X1M1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Thermotoga maritima 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?