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Literature summary extracted from

  • Okita, N.; Ashizawa, D.; Ohta, R.; Abe, H.; Tanuma, S.
    Discovery of novel poly(ADP-ribose) glycohydrolase inhibitors by a quantitative assay system using dot-blot with anti-poly(ADP-ribose) (2010), Biochem. Biophys. Res. Commun., 392, 485-489.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.143 analysis non-radioactive and quantitative assay system for PARG activity based on dot-blot assay using anti-poly(ADP-ribose) and nitrocellulose membrane. The maximum velocity Vmax and the Michaelis constant Km of PARG reaction according to this method are 4.46 microM and 128.33 micromol/min/mg, respectively Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.143 adenosine diphosphate (hydroxymethyl)pyrrolidinediol
-
 Bos taurus
3.2.1.143 eosine Y
-
 Bos taurus
3.2.1.143 phloxine B
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.143 0.00446
-
 Poly(ADP-ribose) 37°C, dot-blot assay Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.143 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.143 commercial preparation
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.143 poly(ADP-ribose) + H2O
-
 Bos taurus ADP-ribose + ADP-ribose oligomer
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.143 0.00066
-
 37°C Bos taurus adenosine diphosphate (hydroxymethyl)pyrrolidinediol
3.2.1.143 0.0019
-
 37°C Bos taurus eosine Y
3.2.1.143 0.005
-
 37°C Bos taurus phloxine B