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Literature summary extracted from

  • Atanassova, A.; Sugita, M.; Sugiura, M.; Pajpanova, T.; Ivanov, I.
    Molecular cloning, expression and characterization of three distinctive genes encoding methionine aminopeptidases in cyanobacterium Synechocystis sp. strain PCC6803 (2003), Arch. Microbiol., 180, 185-193.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.18 genes map-1, map-2, and map-3, encoding three isozymes, DNA and amino acid sequence determination and analysis, expression as His-tagged enzymes in Escherichia coli Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.18 EDTA
-
 Synechocystis sp.
3.4.11.18 H2O2
-
 Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.18 1.5
-
 Met-Ala-Ala recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 1.9
-
 Met-Gly-Gly recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 2.1
-
 Met-Ala-Ala recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 2.3
-
 Met-Gly-Gly recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 5.2
-
 Met-Ala-Ala recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 5.8
-
 Met-Gly-Gly recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.18 Co2+ activates Synechocystis sp.
3.4.11.18 Mg2+ activates Synechocystis sp.
3.4.11.18 Mn2+ activates Synechocystis sp.
3.4.11.18 additional information activating potencies of divalent metal ions with the three isozymes, overview Synechocystis sp.
3.4.11.18 Zn2+ activates Synechocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.18 Synechocystis sp.
-
 strain PCC6803, three isozymes encoded by lr0786 or map-1, slr0918 or map-2, and sll0555 or map-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.18 recombinant His-tagged isozymes from Escherichia coli by nickel affinity chromatography and gel filtration Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.18 Met-Ala-Ala + H2O
-
 Synechocystis sp. Met + Ala-Ala
-
 ?
3.4.11.18 Met-Ala-Met + H2O
-
 Synechocystis sp. Met + Ala-Met
-
 ?
3.4.11.18 Met-Ala-Ser + H2O
-
 Synechocystis sp. Met + Ala-Ser
-
 ?
3.4.11.18 Met-Arg-Phe + H2O substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia Synechocystis sp. Met + Arg-Phe
-
 ?
3.4.11.18 Met-Asp-Gly + H2O substrate of isozyme c3MetAP-Ib, no activity with the other two isozymes Synechocystis sp. Met + Asp-Gly
-
 ?
3.4.11.18 Met-Gly-Gly + H2O
-
 Synechocystis sp. Met + Gly-Gly
-
 ?
3.4.11.18 Met-Leu-Gly + H2O substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia Synechocystis sp. Met + Leu-Gly
-
 ?
3.4.11.18 Met-Met-Ala + H2O substrate of isozymes c1MetAP-Ia and c3MetAP-Ib, no activity with isozyme c2MetAP-Ia Synechocystis sp. Met + Met-Ala
-
 ?
3.4.11.18 additional information substrate specificities of recombinant isozymes, overview, no activity towards tripeptides in which the N-terminal amino acid is not Met, no activity with Pro-Met and X-Gly-Gly peptides Synechocystis sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.18 c1MetAP-Ia
-
 Synechocystis sp.
3.4.11.18 c2MetAP-Ia
-
 Synechocystis sp.
3.4.11.18 c3MetAP-Ib
-
 Synechocystis sp.
3.4.11.18 MetAP
-
 Synechocystis sp.
3.4.11.18 methionine aminopeptidase
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.18 37
-
 all isozymes Synechocystis sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.18 25 50
-
 Synechocystis sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.18 15.25
-
 Met-Gly-Gly recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 15.57
-
 Met-Ala-Ala recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 19.87
-
 Met-Gly-Gly recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 20.02
-
 Met-Ala-Ala recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 21.87
-
 Met-Gly-Gly recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 22.07
-
 Met-Ala-Ala recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.18 7
-
 isozyme c2MetAP-Ia Synechocystis sp.
3.4.11.18 7.5
-
 isozyme c3MetAP-Ib Synechocystis sp.
3.4.11.18 8
-
 isozyme c1MetAP-Ia Synechocystis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.18 6.5 8
-
 Synechocystis sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.18 2.63
-
 Met-Gly-Gly recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 3
-
 Met-Ala-Ala recombinant isozyme c2MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 8.63
-
 Met-Gly-Gly recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 9.53
-
 Met-Ala-Ala recombinant isozyme c1MetAP-Ia, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 11.52
-
 Met-Gly-Gly recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.
3.4.11.18 14.72
-
 Met-Ala-Ala recombinant isozyme c3MetAP-Ib, pH 7.2, 37°C Synechocystis sp.