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Literature summary extracted from
- Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus (2009), Arch. Biochem. Biophys., 488, 109-120.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.8.1 | additional information | heat-induced activation at 60°C with methyl paraoxon as substrate | Geobacillus stearothermophilus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | from pLybAC11 vector overexpressed in Escherichia coli driven from the Escherichia coli rhamnose (rhaBAD) promoter. Plasmid bearing the GsP gene was transformed into Escherichia coli strain BW25113 | Geobacillus stearothermophilus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | in the presence of Co2+, by vapor diffusion method in hanging drop, to 2.36 A resolution. The active site contains three substrate binding pockets: the small (F28, Y30, T70, C74, V268, and W271), large (R230, I233, M236, V237, and W289) and leaving group (Y100 and E103) pockets. Metal binding site contains a tyrosine residue at position 97 in close proximity to the beta-metal | Geobacillus stearothermophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | 0.27 | - |
methyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus |  |
| 3.1.8.1 | 2.1 | - |
ethyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
| 3.1.8.1 | 6.1 | - |
demeton-S | at 35°C, pH 7.2 | Geobacillus stearothermophilus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.8.1 | Co2+ | activity is highest with Co2+ present in the active site | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 36000 | - |
x * 36000, SDS-PAGE | Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.8.1 | Geobacillus stearothermophilus | - |
- |
- |
| 3.1.8.1 | Geobacillus stearothermophilus 10 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.8.1 | in the presence of Co2+, by centrifugation and on Co2+ affinity resin, to over 95% | Geobacillus stearothermophilus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.8.1 | 4°C, 1 month | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.8.1 | demeton-S + H2O | - |
Geobacillus stearothermophilus | 2-ethylsulfanyl-ethanethiol + diethyl phosphate | - |
? | |
| 3.1.8.1 | demeton-S + H2O | - |
Geobacillus stearothermophilus 10 | 2-ethylsulfanyl-ethanethiol + diethyl phosphate | - |
? | |
| 3.1.8.1 | ethyl paraoxon + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + diethyl phosphate | - |
? | |
| 3.1.8.1 | ethyl paraoxon + H2O | - |
Geobacillus stearothermophilus 10 | 4-nitrophenol + diethyl phosphate | - |
? | |
| 3.1.8.1 | methyl paraoxon + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + dimethyl phosphate | - |
? | |
| 3.1.8.1 | methyl paraoxon + H2O | - |
Geobacillus stearothermophilus 10 | 4-nitrophenol + dimethyl phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.8.1 | ? | x * 36000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.8.1 | 25 | 90 | with methyl paraoxon as a substrate, retains full activity after incubation for 15 min between 25-90°C. Can withstand an incubation temperature of 60°C for two days, and retains 72% of its activity after incubation at 90°C for 2 h. The melting temperature is 106.6°C | Geobacillus stearothermophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | 0.0006 | - |
demeton-S | at 35°C, pH 7.2 | Geobacillus stearothermophilus | |
| 3.1.8.1 | 0.00783 | - |
methyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
| 3.1.8.1 | 0.115 | - |
ethyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.8.1 | 0.0000983 | - |
demeton-S | at 35°C, pH 7.2 | Geobacillus stearothermophilus | |
| 3.1.8.1 | 0.029 | - |
methyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
| 3.1.8.1 | 0.05467 | - |
ethyl paraoxon | at 35°C, pH 9.0 | Geobacillus stearothermophilus | |
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