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Literature summary extracted from

  • Koseki, T.; Mochizuki, K.; Kisara, H.; Miyanaga, A.; Fushinobu, S.; Murayama, T.; Shiono, Y.
    Characterization of a chimeric enzyme comprising feruloyl esterase and family 42 carbohydrate-binding module (2010), Appl. Microbiol. Biotechnol., 86, 155-161.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 biotechnology chimeric enzyme FaeA/Aspergillus kawachii family 42 carbohydrate-binding module as an innovative enzymatic tool for biotechnological applications and biotransformation of plant biomass Aspergillus awamori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 chimeric gene of FaeA and Aspergillus kawachii family 42 carbohydrate-binding module (CBM42) cloned in pPICZalphaC vector. Native and chimeric enzyme comprising FaeA-CBM42 and the Saccharomyces cerevisiae alpha-factor secretion signal peptide expressed in Pichia pastoris strain GS115 Aspergillus awamori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 35000
-
 x * 53000, chimeric enzyme, SDS-PAGE. x * 35000, native enzyme, SDS-PAGE Aspergillus awamori
3.1.1.73 53000
-
 x * 53000, chimeric enzyme, SDS-PAGE. x * 35000, native enzyme, SDS-PAGE Aspergillus awamori

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus awamori
-
-
-
3.1.1.73 Aspergillus awamori NBRC4033
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 native and chimeric enzyme, by anion-exchange chromatography and gel filtration Aspergillus awamori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 additional information
-
 0.098 micromol/min/nmol, native enzyme with methyl ferulate as substrate, at 37°C. 0.17 micromol/min/nmol, chimeric enzyme with methyl ferulate as substrate, at 37°C Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 alpha-naphthyl butyrate + H2O
-
 Aspergillus awamori alpha-naphthol + butyrate
-
 ?
3.1.1.73 alpha-naphthyl butyrate + H2O
-
 Aspergillus awamori NBRC4033 alpha-naphthol + butyrate
-
 ?
3.1.1.73 arabinoxylan + H2O CBM42 binds to the arabinofuranose-containing side chain moiety of arabinoxylan. Release of ferulic acid from insoluble arabinoxylan by the chimeric enzyme is approximately 4fold higher than that achieved by FaeA alone after an incubation of 12 h. Chimeric enzyme and xylanase act synergistically for the degradation of arabinoxylan. Arabinoxylan degradation by xylanase is significant when arabinoxylan is pretreated with Aspergillus kawachii family 54 arabinofuranosidase (Abf54), FaeA, and chimeric FaeA-CBM42 than when it is not Aspergillus awamori ferulic acid + ?
-
 ?
3.1.1.73 arabinoxylan + H2O CBM42 binds to the arabinofuranose-containing side chain moiety of arabinoxylan. Release of ferulic acid from insoluble arabinoxylan by the chimeric enzyme is approximately 4fold higher than that achieved by FaeA alone after an incubation of 12 h. Chimeric enzyme and xylanase act synergistically for the degradation of arabinoxylan. Arabinoxylan degradation by xylanase is significant when arabinoxylan is pretreated with Aspergillus kawachii family 54 arabinofuranosidase (Abf54), FaeA, and chimeric FaeA-CBM42 than when it is not Aspergillus awamori NBRC4033 ferulic acid + ?
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus awamori caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus awamori NBRC4033 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus awamori ferulic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus awamori NBRC4033 ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Aspergillus awamori p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Aspergillus awamori NBRC4033 p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl sinapate + H2O
-
 Aspergillus awamori sinapic acid + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 53000, chimeric enzyme, SDS-PAGE. x * 35000, native enzyme, SDS-PAGE Aspergillus awamori

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAEA
-
 Aspergillus awamori
3.1.1.73 feruloyl esterase
-
 Aspergillus awamori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 45 50 native and chimeric enzymes retain more than 90% of their activity after incubation at 45°C and 50°C for 30 min, respectively Aspergillus awamori
3.1.1.73 55
-
 after incubation at 55°C, the chimeric enzyme retains 40% of its activity, whereas, the native enzyme almost loses its activity Aspergillus awamori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 3 11 the chimeric enzyme does not degrade after an incubation of 12 h at room temperature Aspergillus awamori