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Literature summary extracted from

  • Zhou, J.; Huang, H.; Meng, K.; Shi, P.; Wang, Y.; Luo, H.; Yang, P.; Bai, Y.; Zhou, Z.; Yao, B.
    Molecular and biochemical characterization of a novel xylanase from the symbiotic Sphingobacterium sp. TN19 (2009), Appl. Microbiol. Biotechnol., 85, 323-333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xynA19, phylogenetic tree, expression of His-tagged XynA19 without the signal peptide sequence in Escherichia coli strains BL21 (DE3) and Top10 Sphingobacterium sp. TN19

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Km for the recombinant enzyme is 17.9 mg/ml Sphingobacterium sp. TN19

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Sphingobacterium sp. TN19
-
 D-xylose + beta-1,4-xylobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Sphingobacterium sp. TN19 D8L2Y2 a symbiotic bacterium isolated from the gut of Batocera horsfieldi larvae, gene xynA19
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant His-tagged XynA19 from Escherichia coli strains Top10 and BL21(DE3) by ultrafiltration and nickel affinity chromatography Sphingobacterium sp. TN19

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 196.9
-
 purified recombinant enzyme Sphingobacterium sp. TN19

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Sphingobacterium sp. TN19 D-xylose + beta-1,4-xylobiose + ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O
-
 Sphingobacterium sp. TN19 D-xylose + beta-1,4-xylobiose + ? hydrolysis products comprise 20.1% xylose and 79.9% xylobiose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 More XynA19 contains a complete GH10 catalytic domain of GH 10 from Ile38 to Ala381, structure homology modeling of the recombinant enzyme, overview Sphingobacterium sp. TN19

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 More XynA19 belongs to glycosyl hydrolase family 10, GH 10 Sphingobacterium sp. TN19
3.2.1.8 xylanase
-
 Sphingobacterium sp. TN19
3.2.1.8 XynA19
-
 Sphingobacterium sp. TN19

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 45
-
-
 Sphingobacterium sp. TN19

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 10 65 the enzyme shows over 65% of its maximum activity at 20-28°C and about 40% at 10°C Sphingobacterium sp. TN19

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 10
-
 purified recombinant enzyme, pH 6.5, 40% remaining activity Sphingobacterium sp. TN19
3.2.1.8 20
-
 purified recombinant enzyme, pH 6.5, 65% remaining activity Sphingobacterium sp. TN19
3.2.1.8 30 50 purified recombinant enzyme, pH 6.5, over 80% remaining activity, above 50°C the enzyme activity decreases rapidly after 10 min preincubation Sphingobacterium sp. TN19

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 603.8
-
 beta-1,4-xylan pH 6.5, 45°C Sphingobacterium sp. TN19

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
-
 Sphingobacterium sp. TN19