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Literature summary extracted from
- Isolation of a thermostable legume chitinase and study on the antifungal activity (2009), Appl. Microbiol. Biotechnol., 85, 313-321.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.14 | Cu2+ | strong inhibition | Phaseolus vulgaris |  |
| 3.2.1.14 | Hg2+ | strong inhibition | Phaseolus vulgaris | |
| 3.2.1.14 | Pb2+ | strong inhibition | Phaseolus vulgaris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 30600 | - |
PAGE | Phaseolus vulgaris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | - |
Phaseolus vulgaris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 68 | - |
pH 5.4, 50°C | Phaseolus vulgaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | colloidal chitin + H2O | - |
Phaseolus vulgaris | N-acetyl-D-glucosamine + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | monomer | 1 * 30600, SDS-PAGE | Phaseolus vulgaris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 40 | 55 | - |
Phaseolus vulgaris |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 58 | - |
stable up to | Phaseolus vulgaris |
| 3.2.1.14 | 85 | - |
2 min, complete inactivation | Phaseolus vulgaris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 5.5 | - |
- |
Phaseolus vulgaris |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.14 | Phaseolus vulgaris | isoelectric focusing | - |
7.6 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | physiological function | enzyme has potent inhibitory action toward fungal species including Botrytis cinerea, Physalospora piricola, Fusarium oxysporum, and Pythium aphanidermatum | Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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