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Literature summary extracted from

  • Luo, H.; Yang, J.; Yang, P.; Li, J.; Huang, H.; Shi, P.; Bai, Y.; Wang, Y.; Fan, Y.; Yao, B.
    Gene cloning and expression of a new acidic family 7 endo-beta-1,3-1,4-glucanase from the acidophilic fungus Bispora sp. MEY-1 (2010), Appl. Microbiol. Biotechnol., 85, 1015-1023.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.73 synthesis over-expression in Pichia pastoris, with a yield of about 1000 U/ml in a 3.7 l fermentor Bispora sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 gene bgl7A, overexpression in Pichia pastoris strain GS115 Bispora sp. MEY-1
3.2.1.73 expression in Pichia pastoris Bispora sp.

General Stability

EC Number General Stability Organism
3.2.1.73 enzyme is highly resistant to both pepsin and trypsin Bispora sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ag+
-
 Bispora sp. MEY-1
3.2.1.4 Co2+
-
 Bispora sp. MEY-1
3.2.1.4 Cr3+
-
 Bispora sp. MEY-1
3.2.1.4 Cu2+
-
 Bispora sp. MEY-1
3.2.1.4 EDTA
-
 Bispora sp. MEY-1
3.2.1.4 Fe3+
-
 Bispora sp. MEY-1
3.2.1.4 Hg2+
-
 Bispora sp. MEY-1
3.2.1.4 Mg2+
-
 Bispora sp. MEY-1
3.2.1.4 Mn2+
-
 Bispora sp. MEY-1
3.2.1.4 additional information BglA7 is highly resistant to both pepsin and trypsin Bispora sp. MEY-1
3.2.1.4 Ni2+
-
 Bispora sp. MEY-1
3.2.1.4 Pb2+
-
 Bispora sp. MEY-1
3.2.1.4 SDS
-
 Bispora sp. MEY-1
3.2.1.4 Zn2+
-
 Bispora sp. MEY-1
3.2.1.73 Ag+ strong inhibition Bispora sp.
3.2.1.73 EDTA strong inhibition Bispora sp.
3.2.1.73 Hg2+ strong inhibition Bispora sp.
3.2.1.73 Sodium dodecyl sulfate strong inhibition Bispora sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 additional information the enzymatic activity is enhanced or not affected by the presence of Na+, K+, Li+, Ca2+, or 2-mercaptoethanol Bispora sp. MEY-1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 48000
-
 x * 48000, deglycosylated Bgl7A, SDS-PAGE, x * 60000-80000, recombinant glycosylated enzyme, SDS-PAGE Bispora sp. MEY-1
3.2.1.73 45170
-
 x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment Bispora sp.
3.2.1.73 48000
-
 x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment Bispora sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.4 additional information Bispora sp. MEY-1 Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Bispora sp. MEY-1 D2DRB6 acidophilic fungus, CGMCC 2500, gene bgl7A
-
3.2.1.73 Bispora sp. D2DRB6
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.4 glycoprotein
-
 Bispora sp. MEY-1
3.2.1.73 no glycoprotein five potential N-glycosylation site Asn-Xaa-Thr/Ser-Zaa, where Zaa is not Pro, are found. Endo H treatment decreases molecular mass from 60000-80000 to 48000, SDS-PAGE Bispora sp.
3.2.1.73 proteolytic modification presence of an N-terminal signal peptide at residues 1-9 Bispora sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant Bgl7A 1.3fold from Pichia pastoris strain GS115 by ion exchange chromatography Bispora sp. MEY-1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 2740
-
 substrate lichenan Bispora sp. MEY-1
3.2.1.4 4040
-
 substrate Hordeum vulgare beta-glucan Bispora sp. MEY-1
3.2.1.73 2740
-
 substrate lichenan, pH 5.0, 60°C Bispora sp.
3.2.1.73 4040
-
 substrtae beta-D-glucan, pH 5.0, 60°C Bispora sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 beta-glucan + H2O from Hordeum vulgare Bispora sp. MEY-1 ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Bispora sp. MEY-1 ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Bispora sp. MEY-1 ?
-
 ?
3.2.1.4 additional information Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan Bispora sp. MEY-1 ?
-
 ?
3.2.1.4 additional information poor activity with laminarin and oat spelt xylan, no activity with Avicel Bispora sp. MEY-1 ?
-
 ?
3.2.1.73 beta-D-glucan + H2O source: barley, best substrate Bispora sp. ? hydrolysis products from barley beta-glucan are 6.8 micromol/ml glucose and 16.3 micromol/ml cellobiose ?
3.2.1.73 carboxymethyl cellulose + H2O 9.8% of the activity with beta-D-glucan Bispora sp. ?
-
 ?
3.2.1.73 lichenan + H2O 72% of the activity with beta-D-glucan Bispora sp. ? hydrolysis products from lichenan are 9.1 micromol/ml glucose and 9.9 micromol/ml cellobiose, and 0.45 micromol/ml cellotetraose ?
3.2.1.73 additional information poor substrates: laminarin, oat spelt xylan. No substrate: avicel Bispora sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 48000, deglycosylated Bgl7A, SDS-PAGE, x * 60000-80000, recombinant glycosylated enzyme, SDS-PAGE Bispora sp. MEY-1
3.2.1.73 ? x * 45170, calculated, x * 60000-80000, SDS-PAGE of recombinant enzyme, x * 48000, SDS-PAGE after Endo H treatment Bispora sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Bgl7A
-
 Bispora sp. MEY-1
3.2.1.4 endo-beta-1,3-1,4-glucanase
-
 Bispora sp. MEY-1
3.2.1.4 More the enzyme belongs to the acidic glycoside hydrolase family 7, GH7 Bispora sp. MEY-1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
-
 Bispora sp. MEY-1
3.2.1.73 60
-
-
 Bispora sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 25 80 activity rpofile, overview Bispora sp. MEY-1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 60
-
 purified recombinant enzyme, 60 min, stable Bispora sp. MEY-1
3.2.1.4 70
-
 purified enzyme, 48% activity remaining after 5 min, 30% after 60 min Bispora sp. MEY-1
3.2.1.73 60
-
 stable for at least 1 h Bispora sp.
3.2.1.73 70
-
 5 min, 48% residual activity, 60 min, 30% residual activity Bispora sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 additional information
-
 recombinant Bgl7A has three activity peaks at pH 1.5, pH 3.5, and pH 5.0, the last is the highest Bispora sp. MEY-1
3.2.1.4 5
-
-
 Bispora sp. MEY-1
3.2.1.73 1.5
-
 three activity peaks at pH 1.5, 3.5, and 5.0. At pH 1.5, 61% of the activity at pH 5.0 Bispora sp.
3.2.1.73 3.5
-
 three activity peaks at pH 1.5, 3.5, and 5.0. At pH 3.5, 91% of the activity at pH 5.0 Bispora sp.
3.2.1.73 5
-
 three activity peaks at pH 1.5, 3.5, and 5.0. Maximum activity at pH 5.0 Bispora sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 1 6 activity rpofile, overview Bispora sp. MEY-1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 1 8 purified recombinant enzyme, stable, over 85% within this range Bispora sp. MEY-1
3.2.1.73 1 8 37°C, 1 h, more than 85% of maximum activity Bispora sp.