Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gogerty, D.S.; Bobik, T.A.
    Formation of isobutene from 3-hydroxy-3-methylbutyrate by diphosphomevalonate decarboxylase (2010), Appl. Environ. Microbiol., 76, 85004-8010.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.1.1.33 biofuel production enzyme establishes a possible route for biological production of petroleum based fuels and plastics by producing isobutene enzymatically Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.33 expressed in Escherichia coli BL21(DE3) cells. Whole cells of Eschercihia coli produced ScMDD with an N-terminal 6xHis tag form isobutene from 3-hydroxy-3-methylbutyrate at a rate 0.154 nmol/h * g cells Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.33 R74H mutant increases specific activity on non-native substrate (3-hydroxy-3-methylbutyrate) by 4.8fold Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.33 44000
-
 SDS-PAGE Saccharomyces cerevisiae
4.1.1.33 44120
-
 calculated from protein sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.33 ATP + 5-diphosphomevalonate Saccharomyces cerevisiae
-
 ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.33 Saccharomyces cerevisiae P32377
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.33 both, the wild-type 6XHis tag ScMDD and the most active mutant R74H ScMDD2 purified by nickel affinity chromatography under anaerobic conditions, 90 to 95% homogenous Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.33 additional information
-
 improved mutants ScMDD1 (I145F) and ScMDD2 (R74H) produce 3 and 5.888 nmol/h * g cells, respectively, which are 19- and 38fold increases compared to the wild type 6xHis ScMDD Saccharomyces cerevisiae
4.1.1.33 0.00000133
-
 wild-type, 6xHis tagged, produced in Escherichia coli, 37°C, pH 7.4 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.33 ATP + 3-hydroxy-3-methylbutyrate
-
 Saccharomyces cerevisiae ADP + isobutene + CO2 isobutene is identified by gas chromatography with flame ionization detection as well as by GC-mass spectrometry. Isobutene is an important commercial chemical used for the synthesis of butyl rubber, terephthalic acid, specialty chemicals, and a gasoline performance additive known as alkylate ?
4.1.1.33 ATP + 5-diphosphomevalonate
-
 Saccharomyces cerevisiae ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.33 diphosphomevalonate decarboxylase
-
 Saccharomyces cerevisiae
4.1.1.33 ScMDD
-
 Saccharomyces cerevisiae
4.1.1.33 ScMDD1 improved mutant I145F Saccharomyces cerevisiae
4.1.1.33 ScMDD2 improved mutant R74H Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.33 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.33 7.4
-
 assay at Saccharomyces cerevisiae