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Literature summary extracted from

  • Shi, P.; Tian, J.; Yuan, T.; Liu, X.; Huang, H.; Bai, Y.; Yang, P.; Chen, X.; Wu, N.; Yao, B.
    Paenibacillus sp. strain E18 bifunctional xylanase-glucanase with a single catalytic domain (2010), Appl. Environ. Microbiol., 76, 3620-3624.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 XynBE18 expression in Escherichia coli strain Bl21(DE3) Paenibacillus sp. E18

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.8 E129A/E236A the naturally occuring enzyme mutant XynBE18 shows also beta-1,3-1,4-glucan hydrolase activity, EC 3.2.1.6. Recombinant XynBE18 shows specificity toward oat spelt xylan and birchwood xylan and barley beta-1,3-1,4-glucan and lichenin, no activity with carboxymethylcellulose or Avicel, overview Paenibacillus sp. E18

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ strong inhibition Paenibacillus sp. E18
3.2.1.8 Cu2+
-
 Paenibacillus sp. E18
3.2.1.8 Fe3+
-
 Paenibacillus sp. E18
3.2.1.8 Hg2+ strong inhibition Paenibacillus sp. E18
3.2.1.8 Ni2+
-
 Paenibacillus sp. E18
3.2.1.8 SDS
-
 Paenibacillus sp. E18
3.2.1.8 Zn2+
-
 Paenibacillus sp. E18

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 additional information no significant effect on the xylanase activity of recombinant XynBE18 by 1-10 mM Na, K, Li, Mg2, Ca2, or EDTA Paenibacillus sp. E18

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Paenibacillus sp. E18 natural mutant XynBE18 can accommodate xylan and beta-1,3-1,4-glucan, but XynE2 is only accessible to xylan, the cleft of XynBE18 containing the active site is larger than that of XynE2 xylose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Paenibacillus sp. E18
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 59.6
-
 recombinant XynBE18, substrate oat spelt xylan Paenibacillus sp. E18
3.2.1.8 61.57
-
 recombinant XynBE18, substrate birchwood xylan Paenibacillus sp. E18

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O natural mutant XynBE18 can accommodate xylan and beta-1,3-1,4-glucan, but XynE2 is only accessible to xylan, the cleft of XynBE18 containing the active site is larger than that of XynE2 Paenibacillus sp. E18 xylose + ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O from birchwood and oat spelt Paenibacillus sp. E18 xylose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 More XynBE18 structure-function relationship, homology modeling and molecular dynamic simulation, overview Paenibacillus sp. E18

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 XynB18
-
 Paenibacillus sp. E18
3.2.1.8 XynE2
-
 Paenibacillus sp. E18

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 37
-
 assay at Paenibacillus sp. E18

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 30 70 profile, overview Paenibacillus sp. E18

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
 assay at Paenibacillus sp. E18

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5.5 12 activity range, profile, overview Paenibacillus sp. E18