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Literature summary extracted from

  • Sallam, A.; Kast, A.; Przybilla, S.; Meiswinkel, T.; Steinbüchel, A.
    Biotechnological process for production of beta-dipeptides from cyanophycin on a technical scale and its optimization (2009), Appl. Environ. Microbiol., 75, 29-38.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.15.6 food industry the production of beta-dipeptides from cyanophycin by cyanophycinase is economically important, because di- and tripeptides are more efficiently utilized than intact proteins or free amino acids, have a greater nutritional value, and are better absorbed Pseudomonas alcaligenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.6 4-(2-aminoethyl) benzenesulfonylfluoride cyanophycinase is strongly inhibited Pseudomonas alcaligenes
3.4.15.6 additional information no inhibition by EDTA; no inhibition by leupeptin Pseudomonas alcaligenes
3.4.15.6 N-bromosuccinimide total inhibition Pseudomonas alcaligenes
3.4.15.6 phenylmethylsulfonyl fluoride cyanophycinase is strongly inhibited Pseudomonas alcaligenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.15.6 extracellular
-
 Pseudomonas alcaligenes
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.15.6 45000
-
 SDS-PAGE Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.15.6 [L-Asp(4-Arg)]n + H2O Pseudomonas alcaligenes
-
 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?
3.4.15.6 [L-Asp(4-Arg)]n + H2O Pseudomonas alcaligenes DIP1
-
 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.6 Pseudomonas alcaligenes
-
-
-
3.4.15.6 Pseudomonas alcaligenes DIP1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.15.6 centrifugation, proteins are concentrated and desalted using a cross-flow polyethersulfone cassette with a cutoff point of 30 kDa, a purification method for cyanophycinase is developed which depended on the strong affinity of the enzyme in the crude extract for its insoluble substrate cyanophycin Pseudomonas alcaligenes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.15.6 culture filtrate
-
 Pseudomonas alcaligenes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.6 [L-Asp(4-Arg)]n + H2O
-
 Pseudomonas alcaligenes [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?
3.4.15.6 [L-Asp(4-Arg)]n + H2O 2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production Pseudomonas alcaligenes [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?
3.4.15.6 [L-Asp(4-Arg)]n + H2O
-
 Pseudomonas alcaligenes DIP1 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?
3.4.15.6 [L-Asp(4-Arg)]n + H2O 2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production Pseudomonas alcaligenes DIP1 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.15.6 CGPase
-
 Pseudomonas alcaligenes
3.4.15.6 CphEal
-
 Pseudomonas alcaligenes
3.4.15.6 cyanophycinase
-
 Pseudomonas alcaligenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.15.6 50
-
-
 Pseudomonas alcaligenes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.15.6 10 80 20 min, sodium phosphate buffer pH 7.0 Pseudomonas alcaligenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.15.6 additional information
-
 maximum degradation activity at 50°C and complete inactivation at 68°C Pseudomonas alcaligenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.15.6 8.5
-
-
 Pseudomonas alcaligenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.15.6 5 9 20 min, 30°C Pseudomonas alcaligenes