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Literature summary extracted from

  • Samra, Z.Q.; Shabir, S.; Rehmat, Z.; Zaman, M.; Nazir, A.; Dar, N.; Athar, M.A.
    Synthesis of cholesterol-conjugated magnetic nanoparticles for purification of human paraoxonase 1 (2010), Appl. Biochem. Biotechnol., 162, 671-686.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.8.1 acetaminophen at 37°C, 5% slightly reduces activity by 18% concentration Homo sapiens
3.1.8.1 acetone at 37°C, 1 mM reduces activity by 82% Homo sapiens
3.1.8.1 acetyl salicylic acid at 37°C, 5% slightly reduces activity by 10% concentration Homo sapiens
3.1.8.1 beta-mercaptoethanol at 37°C, 1 mM reduces activity by 52% Homo sapiens
3.1.8.1 Cd2+ at 37°C, 1 mM inhibits activity Homo sapiens
3.1.8.1 Co2+ at 37°C, 1 mM reduces activity by 30% Homo sapiens
3.1.8.1 Cu2+ at 37°C, 1 mM inhibits activity Homo sapiens
3.1.8.1 dimethyl sulfoxide at 37°C, 5% completely inhibits activity Homo sapiens
3.1.8.1 dimethylformamide at 37°C, 5% completely inhibits activity Homo sapiens
3.1.8.1 ethanol at 37°C, 1 mM reduces activity by 43% Homo sapiens
3.1.8.1 gentamicin sulfate at 37°C, 0.6 mg/ml reduces activity by 50% Homo sapiens
3.1.8.1 iodoacetic acid at 37°C, 5% completely inhibits activity Homo sapiens
3.1.8.1 kanamycin sulfate at 37°C, 0.6 mg/ml reduces activity by 50% Homo sapiens
3.1.8.1 additional information at 37°C, 1 mM Mg2+, 1 mM Mn2+, 5% glycerol, 5% PEG 6000, 5% ammonium sulfate, and 5% phenylmethylsulfonyl fluoride do not inhibit the enzyme activity Homo sapiens
3.1.8.1 Ni2+ at 37°C, 1 mM inhibits activity Homo sapiens
3.1.8.1 Pb2+ at 37°C, 1 mM reduces activity by 35% Homo sapiens
3.1.8.1 sodium ampicillin at 37°C, 30 mg/ml reduces activity by 50% Homo sapiens
3.1.8.1 Sodium dodecyl sulfate at 37°C, 5% completely inhibits activity Homo sapiens
3.1.8.1 streptomycin sulfate at 37°C, 0.6 mg/ml reduces activity by 50% Homo sapiens
3.1.8.1 Zn2+ at 37°C, 1 mM reduces activity by 70% Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.74
-
 phenyl acetate 2 mM substrate, at pH 8.0 and 37°C Homo sapiens
3.1.8.1 1.3
-
 paraoxon 2 mM substrate, at pH 8.0 and 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.8.1 additional information at 37°C, 1 mM Mg2+ and Mn2+ have no stimulatory effect on activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 45000
-
 x * 45000, SDS-PAGE Homo sapiens
3.1.8.1 45000
-
 x * 45000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens
-
-
-
3.1.8.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 by Triton-X-100-treatment, ammonium sulfate precipitation, cholesterol-conjugated magnetic nanoparticles and gel filtration, at 4°C, to homogeneity, 515fold with 73% yield Homo sapiens
3.1.8.1 by Triton-X-100-treatment, ammonium sulfate precipitation, cholesterol-conjugated magnetic nanoparticles and gel filtration, at 4°C, to homogeneity, 515fold with 73% yield Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 plasma
-
 Homo sapiens
-
3.1.8.1 plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.8.1 0.85
-
 plasma, at 37°C Homo sapiens
3.1.8.1 438.2
-
 515fold purified enzyme, at 37°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.8.1 4°C, 20% glycerol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 phenyl acetate + H2O
-
 Homo sapiens phenol + acetate
-
 ?
3.1.8.1 paraoxon + H2O
-
 Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 ? x * 45000, SDS-PAGE Homo sapiens
3.1.8.1 ? x * 45000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 paraoxonase 1
-
 Homo sapiens
3.1.8.1 PON1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.2 37
-
-
 Homo sapiens
3.1.8.1 37
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.8.1 37
-
 is stable for 8 to 10 h at 37°C. Completely loses activity at 50°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.2 8
-
-
 Homo sapiens
3.1.8.1 8
-
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.8.1 additional information
-
 activity is reduced below pH 7.0 and at alkaline pH Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.2 Homo sapiens purified PON1, isoelectric focusing
-
 5.3
3.1.8.1 Homo sapiens purified PON1, isoelectric focusing
-
 5.3