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Literature summary extracted from

  • Li, Y.; Zhang, B.; Chen, X.; Chen, Y.; Cao, Y.
    Improvement of Aspergillus sulphureus endo-beta-1,4-xylanase expression in Pichia pastoris by codon optimization and analysis of the enzymic characterization (2010), Appl. Biochem. Biotechnol., 160, 1321-1331.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xynB, constitutive expression of wild-type enzyme and enzyme without signal sequence in Pichia pastoris strain X-33, the recombinant enzyme is secreted to the culture medium, nmethod optimization, overview Aspergillus sulphureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+
-
 Aspergillus sulphureus
3.2.1.8 Mn2+
-
 Aspergillus sulphureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Michaelis-Menten kinetics Aspergillus sulphureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 additional information no or poor inhibition by EDTA, Na+, K+, Ca2+, Mg2+, and Fe2+, overview Aspergillus sulphureus
3.2.1.8 Zn2+ activates Aspergillus sulphureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O Aspergillus sulphureus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus sulphureus Q3S401 gene xynB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O
-
 Aspergillus sulphureus ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O oat spelt xylan, degradation into xylo-oligosaccharides of various lengths Aspergillus sulphureus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endo-beta-1,4-xylanase
-
 Aspergillus sulphureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
 recombinant enzyme Aspergillus sulphureus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 20 70 50% of maximal activity at 20°C, 20% at 70°C Aspergillus sulphureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 purified recombinant enzyme, half-life is 30 min Aspergillus sulphureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 6551
-
 beta-1,4-xylan recombinant wild-type enzyme, pH 5.0, 50°C, oat spelt xylan Aspergillus sulphureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
 recombinant enzyme Aspergillus sulphureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 2.4 7.4 recombinant enzyme, rapid decrease in activity above pH 6.4, profile, overview Aspergillus sulphureus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 2.4
-
 purified recombinant enzyme, over 90% activity remaining after 2 h Aspergillus sulphureus